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Anti-Apolipoprotein E抗体[E6D7] (ab1907)

  • Datasheet
  • SDS
Reviews (7)Q&A (3)References (12)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [E6D7] (ab1907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein E antibody [E6D7] (ab1907)

Key features and details

  • Mouse monoclonal [E6D7] to Apolipoprotein E
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Apolipoprotein E抗体[E6D7]
    参阅全部 Apolipoprotein E 一抗
  • 描述

    小鼠单克隆抗体[E6D7] to Apolipoprotein E
  • 宿主

    Mouse
  • 经测试应用

    适用于: IHC-P, ICC/IFmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Apolipoprotein E. Spans the polymorphic amino acid position 158.

  • 阳性对照

    • IHC-P: Human liver tissue. ICC/IF: HepG2 cells. WB: Human THP-1 macrophage whole cell lysate
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 20/05/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 克隆

    单克隆
  • 克隆编号

    E6D7
  • 同种型

    IgG1
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
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    • Neurology process
    • Neurodegenerative disease
    • Other
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相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Apolipoprotein E (ab55210)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab1907于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (2)
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.

靶标

  • 功能

    Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
  • 组织特异性

    Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.
  • 疾病相关

    Defects in APOE are a cause of hyperlipoproteinemia type 3 (HLPP3) [MIM:107741]; also known as familial dysbetalipoproteinemia. Individuals with HLPP3 are clinically characterized by xanthomas, yellowish lipid deposits in the palmar crease, or less specific on tendons and on elbows. The disorder rarely manifests before the third decade in men. In women, it is usually expressed only after the menopause. The vast majority of the patients are homozygous for APOE*2 alleles. More severe cases of HLPP3 have also been observed in individuals heterozygous for rare APOE variants. The influence of APOE on lipid levels is often suggested to have major implications for the risk of coronary artery disease (CAD). Individuals carrying the common APOE*4 variant are at higher risk of CAD.
    Genetic variations in APOE are associated with Alzheimer disease type 2 (AD2) [MIM:104310]. It is a late-onset neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death. Note=The APOE*4 allele is genetically associated with the common late onset familial and sporadic forms of Alzheimer disease. Risk for AD increased from 20% to 90% and mean age at onset decreased from 84 to 68 years with increasing number of APOE*4 alleles in 42 families with late onset AD. Thus APOE*4 gene dose is a major risk factor for late onset AD and, in these families, homozygosity for APOE*4 was virtually sufficient to cause AD by age 80. The mechanism by which APOE*4 participates in pathogenesis is not known.
    Defects in APOE are a cause of sea-blue histiocyte disease (SBHD) [MIM:269600]; also known as sea-blue histiocytosis. This disorder is characterized by splenomegaly, mild thrombocytopenia and, in the bone marrow, numerous histiocytes containing cytoplasmic granules which stain bright blue with the usual hematologic stains. The syndrome is the consequence of an inherited metabolic defect analogous to Gaucher disease and other sphingolipidoses.
    Defects in APOE are a cause of lipoprotein glomerulopathy (LPG) [MIM:611771]. LPG is an uncommon kidney disease characterized by proteinuria, progressive kidney failure, and distinctive lipoprotein thrombi in glomerular capillaries. It mainly affects people of Japanese and Chinese origin. The disorder has rarely been described in Caucasians.
  • 序列相似性

    Belongs to the apolipoprotein A1/A4/E family.
  • 翻译后修饰

    Synthesized with the sialic acid attached by O-glycosidic linkage and is subsequently desialylated in plasma. O-glycosylated with core 1 or possibly core 8 glycans. Thr-307 is a minor glycosylation site compared to Ser-308.
    Glycated in plasma VLDL of normal subjects, and of hyperglycemic diabetic patients at a higher level (2-3 fold).
    Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位

    Secreted.
  • Target information above from: UniProt accession P02649 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 348 Human
    • Omim: 107741 Human
    • SwissProt: P02649 Human
    • Unigene: 654439 Human
    • 别名

      • AD2 antibody
      • Apo-E antibody
      • APOE antibody
      • APOE_HUMAN antibody
      • APOEA antibody
      • Apolipoprotein E antibody
      • Apolipoprotein E3 antibody
      • ApolipoproteinE antibody
      • Apoprotein antibody
      • LDLCQ5 antibody
      • LPG antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [E6D7] (ab1907)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [E6D7] (ab1907)

      Ab1907 staining Human liver. Staining is localised to cytoplasmic and extracelular regions.
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein E antibody [E6D7] (ab1907)
      Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein E antibody [E6D7] (ab1907)

      ICC/IF image of ab1907 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1907, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      This image was generated using the ascites version of the product.

    实验方案

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (12)

    发表研究结果有使用 ab1907?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1907 被引用在 12 文献中.

    • Xiang J  et al. Melatonin-induced ApoE expression in mouse astrocytes protects endothelial cells from OGD-R induced injuries. Transl Psychiatry 10:181 (2020). PubMed: 32513932
    • Taylor X  et al. A1 reactive astrocytes and a loss of TREM2 are associated with an early stage of pathology in a mouse model of cerebral amyloid angiopathy. J Neuroinflammation 17:223 (2020). PubMed: 32711525
    • Hirose S  et al. Apolipoprotein E binds to and reduces serum levels of DNA-mimicking, pyrrolated proteins. J Biol Chem 294:11035-11045 (2019). PubMed: 31167785
    • Bhowmik M  et al. Pilot-Scale Study Of Human Plasma Proteomics Identifies ApoE And IL33 As Markers In Atopic Asthma. J Asthma Allergy 12:273-283 (2019). PubMed: 31571934
    • Muñoz-Cabrera JM  et al. Bexarotene therapy ameliorates behavioral deficits and induces functional and molecular changes in very-old Triple Transgenic Mice model of Alzheimer´s disease. PLoS One 14:e0223578 (2019). PubMed: 31596896
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 10 Abreviews or Q&A

    Western blot abreview for Anti-Apolipoprotein E antibody [E6D7]

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    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Melanoma cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    Melanoma cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 16 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Apolipoprotein E antibody [E6D7]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    No
    Specification
    Lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Yong Sun Lee

    Verified customer

    提交于 Jul 03 2018

    Western blot abreview for Anti-Apolipoprotein E antibody [E6D7]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (1 lane : A549 / 2 lane : H460 human lung cancer c)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    30 µg
    Specification
    1 lane : A549 / 2 lane : H460 human lung cancer c
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Yong Sun Lee

    Verified customer

    提交于 May 22 2018

    Western blot abreview for Anti-Apolipoprotein E antibody [E6D7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human THP-1 macrophages)
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Loading amount
    70 µg
    Treatment
    100 µg/ml oxidized LDL (oxLDL) +/- 10 µg/ml apoA-I
    Specification
    Human THP-1 macrophages
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Anca Sima

    Verified customer

    提交于 Jul 03 2015

    Western blot abreview for Anti-Apolipoprotein E antibody [E6D7]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain lysate)
    Loading amount
    20 µg
    Specification
    Brain lysate
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Ruma Raha-Chowdhury

    Verified customer

    提交于 Aug 13 2012

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Apolipoprotein E antibody [E6D7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Cell (Brain section)
    Specification
    Brain section
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% TritonX in 0.1% PBS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Ruma Raha-Chowdhury

    Verified customer

    提交于 Aug 07 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Apolipoprotein E antibody [E6D7]

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Thyroid)
    Specification
    Thyroid
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.1%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 29 2007

    Question

    Hi

    Does antibody ab1907 react with rat tissue?

    Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Dec 19 2012

    Answer

    Thank you for contacting us and for your interest in our products.

    I am sorry to confirm that as far as we are aware, ab1907 Apolipoprotein E antibody [E6D7] has not yet been tested with samples from rat. All tested species covered by the 6 month guarantee are stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

    I have checked the alignment of the immunogen with the rat protein, this is around 75%. We would usually suggest that alignment shoudl be over 85% to predict that an antibody will detect in a different species. The alignment with the rat is still reasonably high in this case and there may be some crossreactivity. However, we are not able to guarantee this without further testing.

    If you would like to test ab1907in rat, please do not hesitaet to contact me again by replying to this message prior to the purchase as you would be eligible for our testing discount program.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

    Read More

    Abcam Scientific Support

    回复于 Dec 19 2012

    Question

    J’aimerais obtenir une précision concernant l’anticorps dirigé contre la protéine ApoE [E6D7]. Il est précisé que l’antigène utilisé recouvre la région polymorphique de l’acide aminé en position 158. Pourriez-vous me préciser quel est l’aa en position 158 utilisé dans le peptide, quel variant de fait de la protéine ApoE peut être reconnu (si spécificité il y a) ?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 27 2012

    Answer

    Merci de nous avoir contactés.

    Je suis vraiment désolée pour le délai de ma réponse, je suis toujours en contact avec notre collaborateur a propos de votre question.

    Cependant, Je peux vous confirmer que l’acide aminé en position 158 est un histidine. Cet anticorps peut reconnaitre les isoforms E2, E3 et E4 de la protéine apolipoprotein E.

    Une fois de plus, je suis désolée pour cette réponse tardive et je vous tiendrai au courant si j’ai d’autres informations à propos de ce sujet.

    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Monoclonal de lapin gratuit pour tout achat d'anticorps primaire, dans la limite des stocks disponibles. Notez le code "RABMAB-XBSMG" sur votre prochaine commande d’anticorps primaire. Pour plus d'informations, visitez le lien suivant: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    回复于 Nov 27 2012

    Question

    I have used ab1907 in western blotting and the expected band at 38kDa does not appear.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 04 2012

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab20874.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 Sep 04 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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    研究类别(A-Z)
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