Anti-Apolipoprotein E抗体[D6E10] - BSA and Azide free (ab1906)

Thank you for contacting us and your interest in our products.

Unfortunately, none of the three antibodies you are interested in have been tested in in both the application you are interested in with rat samples and we do not have any other antibodies against Apolipoprotein E which have either.

Ab7620 has been shown to detect the rat Apolipoprotein E, but has not been used in IHC. Ab1906 has been used in IHC with paraffin embedded sections but not with tissue from rat. From the sequence homology of the immunogen used I would also suggest that this anitbody would be unlikely to cross react with protein from rat. Finally, ab20874 has been used with rat samples and IHC with frozen sections, but not in paraffin embedded sections. We cannot therefore guarantee how any of these antibodies would perform in your experiment. I would suggest that ab23832 may be the most suitable to try to see if it would be suitable for your experiment.

We do however have an Abtrial program which may be of interest to you. This offer means that if you purchase ab23832 as normal, test the antibody with your rat samples in IHC-P and let us know of the results through an Abreview (no matter whether positive or negative) you would then be eligible to claim the value of ab23832 off any product in our catalogue. More information on this offer can be found here:

www.abcam.com/abtrial

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab23832.

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

Thank you for getting back to me.

This is to let you know that I have placed a new order for you - for one vial of ab1906 as a free of charge replacement and the new order number is 1206066.

I hope the second vial will work as it is expected, and please do let me know how you are getting on with this product.

Thank you for getting back to us with the updates.

I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

Currently we have new batch in our system so I can offer your troubled customer a free of charge replacement and a credit note which can be used in the next order.

If you need any further assistance in the future, please do not hesitate to contact me.

Thank you for contacting us and for sending the questionnaire. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. The only lot of ab17929 we have in stock is the same as the one metioned in the questionaaire. Therefore, as requested, I have issued a free of charge replacement with one unit of ab20874. This free of charge replacement was added to the order number ******.

To check the status of the order please contact our Customer Service team and reference this number.

I looked at the protocol and I would like to suggest:
- to load a maximum of 30µg on the gel. Too much protein usually brings a lot of background.
- to dilute the primary antibody more (1/1000 is commonly used), too much antibody usually brings a lot of background too.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

Thank you for your enquiry regardingab1906 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer ishaving problems with this antibody.

After reading through the detailed protocol you kindly forwarded to Abcam, I would like to make the following comments/suggestions:

1) Blocking:

I would suggest increasing the concentration of the blocking agent from 1% to 5% and using this solution for diluting the primary and the secondary antibody as well.

2) Dilution of the primary antibody:

It may be worth diluting the primary antibody further ie. 1/750 and 1/1000.

3) No primary control:

Does the detection system work fine? Have you used it successfully with another primary antibody? Have you run a no primary - only secondary antibody - control to see if any of the non-specific bands are due to the secondary or not? If you have not done yet, I would advise you to check it.

I hope this will be useful for you. Should your customerstill have any problem with this antibody after following these suggestions, then please confirm the batch number and the Abcam Order Number and do not hesitate to contact our Technical Department again.

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem.

1) Could you provide some further details of the protocol used and complete the following form (attached as a word document). I am particularly interested in the following information:

- sample preparation,

- lysis buffer used,

- specification of the secondary antibody (host species, specificity),

- positive control applied

2) It would be much appreciated if you could attach a full size WBimage with molecular marker bands (MW) to the response. Please also describe what the different lanes represent.

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.