重组Anti-Apolipoprotein CIII抗体[EPR1371(2)] (ab108205)
![Western blot - Anti-Apolipoprotein CIII antibody [EPR1371(2)] (ab108205)](https://www.abcam.cn/ps/products/108/ab108205/Images/ab108205-120708-anti-apolipoprotein-ciii-antibody-epr13712-western-blot.gif "Western blot - Anti-Apolipoprotein CIII antibody [EPR1371(2)] (ab108205)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1371(2)] to Apolipoprotein CIII
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Apolipoprotein CIII抗体[EPR1371(2)]
参阅全部 Apolipoprotein CIII 一抗 -
描述
兔单克隆抗体[EPR1371(2)] to Apolipoprotein CIII -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: lysate from Human plasma or HDL cells; IHC-P: Human liver tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1371(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108205于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
|
|
| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
靶标
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功能
Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. -
组织特异性
Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine. -
疾病相关
Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL). -
序列相似性
Belongs to the apolipoprotein C3 family. -
翻译后修饰
O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 345 Human
- Omim: 107720 Human
- SwissProt: P02656 Human
- Unigene: 73849 Human
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别名
- APOC3 antibody
- APO C3 antibody
- Apo CIII antibody
see all
图片
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Western blot - Anti-Apolipoprotein CIII antibody [EPR1371(2)] (ab108205)All lanes : Anti-Apolipoprotein CIII antibody [EPR1371(2)] (ab108205) at 1/1000 dilution
Lane 1 : Human plasma
Lane 2 : HDL cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein CIII antibody [EPR1371(2)] (ab108205)](https://www.abcam.cn/ps/products/108/ab108205/Images/ab108205-120707-anti-apolipoprotein-ciii-antibody-epr13712-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein CIII antibody [EPR1371(2)] (ab108205)ab108205 at 1/250 dilution staining Apolipoprotein CIII in paraffin-embedded Human liver tissue by immunohistochemistry,.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Apolipoprotein CIII antibody [EPR1371(2)] (ab108205)](https://www.abcam.cn/ps/products/108/ab108205/Images/ab108205-3-benefits-of-recombinant-antibodies.png)
Anti-Apolipoprotein CIII antibody [EPR1371(2)] (ab108205)
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab108205 被引用在 2 文献中.
- Meng Y et al. KIAA1429 facilitates progression of hepatocellular carcinoma by modulating m6A levels in HPN. Heliyon 9:e22084 (2023). PubMed: 38058614
- Arya R et al. Serum Small Extracellular Vesicles Proteome of Tuberculosis Patients Demonstrated Deregulated Immune Response. Proteomics Clin Appl 14:e1900062 (2020). PubMed: 31532894