重组Anti-Apolipoprotein CIII抗体[EP1372Y] (ab76305)
![Western blot - Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305)](https://www.abcam.cn/ps/products/76/ab76305/Images/ab76305-3-anti-apolipoprotein-ciii-antibody-ep1372y-western-blot-plasma-serum-lysates-human.jpg "Western blot - Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1372Y] to Apolipoprotein CIII
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Apolipoprotein CIII抗体[EP1372Y]
参阅全部 Apolipoprotein CIII 一抗 -
描述
兔单克隆抗体[EP1372Y] to Apolipoprotein CIII -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human plasma and serum lysates
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常规说明
The production method for this product has been changed from hybridoma to recombinant on 18th June 2024.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1372Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76305于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
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| 说明 |
|---|
|
WB
1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
靶标
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功能
Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. -
组织特异性
Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine. -
疾病相关
Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL). -
序列相似性
Belongs to the apolipoprotein C3 family. -
翻译后修饰
O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 345 Human
- Omim: 107720 Human
- SwissProt: P02656 Human
- Unigene: 73849 Human
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别名
- APOC3 antibody
- APO C3 antibody
- Apo CIII antibody
see all
图片
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Western blot - Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305)All lanes : Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305) at 1/10000 dilution
Lane 1 : Human plasma lysate
Lane 2 : Human serum lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution
Predicted band size: 11 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1, 3 secnods; Lane 2, 140 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab76305 被引用在 5 文献中.
- Liu K et al. Multiomics analysis of naturally efficacious lipid nanoparticle coronas reveals high-density lipoprotein is necessary for their function. Nat Commun 14:4007 (2023). PubMed: 37414857
- Li L et al. Proteomics analysis of potential serum biomarkers for insulin resistance in patients with polycystic ovary syndrome. Int J Mol Med 45:1409-1416 (2020). PubMed: 32323743
- Lv P et al. Apolipoprotein C-III in the high-density lipoprotein proteome of cerebral lacunar infarction patients impairs its anti-inflammatory function. Int J Mol Med 41:61-68 (2018). PubMed: 29115584
- Zhang H et al. Characterization of apolipoprotein C1 in hepatitis C virus infection and morphogenesis. Virology 524:1-9 (2018). PubMed: 30130702
- Wang Y et al. Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman primate serum. J Lipid Res 52:1265-71 (2011). PubMed: 21371998