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AB21032

Anti-Apolipoprotein CIII 抗体

Anti-Apolipoprotein CIII antibody

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(5 Publications)

Anti-Apolipoprotein CIII antibody (ab21032) is a rabbit polyclonal antibody detecting Apolipoprotein CIII in Western Blot, ICC/IF, ELISA. Suitable for Human, Monkey.

- Trusted since 2006

查看别名

Apolipoprotein C-III, Apo-CIII, ApoC-III, Apolipoprotein C3, APOC3

2 Images
Western blot - Anti-Apolipoprotein CIII antibody (AB21032)
  • WB

Unknown

Western blot - Anti-Apolipoprotein CIII antibody (AB21032)

All lanes:

Western blot - Anti-Apolipoprotein CIII antibody (ab21032) at 1 µg/mL

Lane 1:

Native Human Apolipoprotein CIII (ab77944) at 0.1 µg

Lane 2:

Native Human Apolipoprotein CIII (ab77944) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 11 kDa

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Exposure time: 1min

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein CIII antibody (AB21032)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein CIII antibody (AB21032)

ICC/IF image of ab21032 stained HepG2 (Human liver hepatocellular carcinoma cell line) cells.

The cells were fixed in 4% formaldehyde for 10 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21032, 5 μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa-Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa-Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour.

DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 μM.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human, Monkey

应用

sELISA, ELISA, WB, ICC/IF

applications

免疫原

Full Length Protein corresponding to Human Apolipoprotein C-III. The exact immunogen used to generate this antibody is proprietary information.

P02656

特异性

Specifically binds to human Apo CIII.

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反应性数据

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AB108205

Anti-Apolipoprotein CIII antibody [EPR1371(2)]

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产品详情

What is this antibody validated in?
Anti-Apolipoprotein CIII antibody (ab21032) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, Monkey samples.

What is the molecular weight of Apolipoprotein CIII?
Anti-Apolipoprotein CIII (ab21032) specifically detects a band for Apolipoprotein CIII (UniProt: P02656) at a molecular weight of 11kDa.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Purified by Human Apo CIII-Sepharose affinity column.
存储溶液
Preservative: 0.02% Sodium azide Constituents: PBS, 0.435% Sodium chloride, 0.0292% EDTA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein CIII also known as Apo C-III or ApoC3 is an apolipoprotein with a molecular mass of approximately 8.8 kDa. It is synthesized in the liver and intestine and circulates in the bloodstream primarily as part of very low-density lipoproteins (VLDL) and high-density lipoproteins (HDL). Its main role involves the regulation of lipid metabolism particularly in relation to triglyceride-rich particles. Apo C-III serves as an inhibitor of lipoprotein lipase an enzyme essential for the hydrolysis of triglycerides into free fatty acids.
Biological function summary

Apolipoprotein CIII plays a pivotal role in lipid and lipoprotein metabolism. It is not part of a large protein complex but interacts with lipoproteins as a modulatory protein. Apo C-III affects triglyceride metabolism by delaying the clearance of VLDL and enhancing hepatic triglyceride secretion. Additionally it modulates the uptake of lipoprotein remnants by liver receptors thereby influencing the plasma levels of triglycerides and cholesterol.

Pathways

Apolipoprotein CIII is involved in the regulation of lipid metabolic pathways. It plays a role in the lipoprotein assembly pathway and the triglyceride biosynthetic process. This protein impacts the interaction between lipoprotein lipase and its substrates hindering enzyme activity which leads to elevated plasma triglyceride levels. It relates closely to other apolipoproteins such as apolipoprotein E which also participates in lipid transport and metabolism although with distinct functions and regulatory mechanisms.

Apolipoprotein CIII is implicated in conditions like hypertriglyceridemia and cardiovascular disease. Elevated levels of Apo C-III contribute to high triglyceride levels promoting atherosclerosis development due to the retention of triglyceride-rich lipoproteins in the bloodstream. This association links it to cardiovascular disease risk where its role contrasts with proteins like apolipoprotein AI which is mainly involved in protective effects via HDL metabolism. Understanding the modulation of Apo C-III levels offers potential therapeutic avenues for managing these lipid-related disorders.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma (PubMed : 18201179, PubMed : 22510806). Plays a multifaceted role in triglyceride homeostasis (PubMed : 18201179, PubMed : 22510806). Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs) (PubMed : 18201179, PubMed : 22510806). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors (PubMed : 18201179, PubMed : 22510806). Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners (PubMed : 18408013).
See full target information Apolipoprotein C-III
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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

European journal of pharmacology 828:60-66 PubMed29555504

2018

Alantolactone suppresses APOC3 expression and alters lipid homeostasis in L02 liver cells.

Applications

Unspecified application

Species

Unspecified reactive species

Meiting Yang,Hanhan Zhao,Huihan Ai,Hongbin Zhu,Shuyue Wang,Yongli Bao,Yuxin Li

Nature medicine 23:1086-1094 PubMed28825717

2017

A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels.

Applications

Unspecified application

Species

Unspecified reactive species

Sumeet A Khetarpal,Xuemei Zeng,John S Millar,Cecilia Vitali,Amritha Varshini Hanasoge Somasundara,Paolo Zanoni,James A Landro,Nicole Barucci,William J Zavadoski,Zhiyuan Sun,Hans de Haard,Ildikó V Toth,Gina M Peloso,Pradeep Natarajan,Marina Cuchel,Sissel Lund-Katz,Michael C Phillips,Alan R Tall,Sekar Kathiresan,Paul DaSilva-Jardine,Nathan A Yates,Daniel J Rader

Proceedings of the National Academy of Sciences of 109:9893-8 PubMed22566642

2012

Probing isoform-specific functions of polypeptide GalNAc-transferases using zinc finger nuclease glycoengineered SimpleCells.

Applications

IP

Species

Human

Katrine T-B G Schjoldager,Sergey Y Vakhrushev,Yun Kong,Catharina Steentoft,Aaron S Nudelman,Nis B Pedersen,Hans H Wandall,Ulla Mandel,Eric P Bennett,Steven B Levery,Henrik Clausen

Neuroscience research 73:161-7 PubMed22426495

2012

Identification and quantification of differentially expressed proteins in plasma of spinocerebellar ataxia type 12.

Applications

Unspecified application

Species

Unspecified reactive species

Vishnu Swarup,Achal K Srivastava,Moganty R Rajeswari

Journal of lipid research 52:1265-71 PubMed21371998

2011

Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman primate serum.

Applications

sELISA

Species

Cynomolgus monkey

Yuxin Wang,Zhili Song,Janice D Wagner,Catherine Pachuk,Romesh R Subramanian
View all publications
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