AB313868

重组Anti-Apolipoprotein B抗体[HL1962] - BSA and Azide free

Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free

Recombinant
了解详情

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal Apolipoprotein B antibody. Carrier free. Suitable for I-ELISA, ICC/IF, WB and reacts with Recombinant fragment, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human APOB.

查看别名

Apolipoprotein B-100, Apo B-100, APOB, Apolipoprotein B100

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free (AB313868)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free (AB313868)

Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells fixed in 4% paraformaldehyde at room temperature for 15 minutes labeling Apolipoprotein B with ab313868 at 1/500 dilution (green). Blue : Fluoroshield with DAPI. Scale bar= 10um.

Indirect ELISA - Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free (AB313868)

I-ELISA

Supplier Data

Indirect ELISA - Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free (AB313868)

Indirect ELISA analysis was performed by coating the plate with purified native human ApoB protein (62.5-4000 ng/mL). Coated protein was probed with ab313868 at 1 ug/mL. Goat anti-rabbit IgG antibody at 1/10000 dilution was used to detect the bound primary antibody.

Supplier Data

Western blot - Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free (AB313868)

All lanes:

Western blot - Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free (ab313868) at 1/1000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Lane 2:

HepG2 conditioned medium at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 516 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

HL1962

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

ICC/IF, I-ELISA, WB

applications

免疫原

Recombinant Fragment Protein within Human APOB.

P04114

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Recombinant fragment": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1 µg/mL", "IELISA-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein B often referred to as ApoB is a core protein component of low-density lipoproteins (LDL) and is essential for their assembly and secretion. ApoB is also known as apolipoproteine B and apolipoprotein B protein. This protein has a large molecular weight approximately 550 kDa. ApoB is primarily expressed in the liver and to some extent in the intestine. Its large size allows it to provide structural integrity to lipoprotein particles playing a role in lipid metabolism.

Biological function summary

ApoB plays a significant role in lipid transport and metabolism. It is a critical component of the lipoprotein particle complex which is responsible for the transport of lipids such as cholesterol and triglycerides through the bloodstream. ApoB helps in the binding of lipoproteins to cell receptors facilitating the uptake of lipids by tissues. Its presence on the surface of lipoproteins makes it important for normal cholesterol homeostasis in the human body.

Pathways

ApoB is integrated within the lipid metabolism pathway. It interacts with proteins such as microsomal triglyceride transfer protein (MTP) during the assembly and secretion of lipoproteins. This pathway is essential for the normal transport of lipids throughout the body and influences processes like absorption of dietary fats. ApoB also plays a role in the pathways related to atherosclerosis where it influences the deposition of cholesterol in arterial walls.

ApoB is well-associated with cardiovascular diseases notably atherosclerosis and familial hypercholesterolemia. In these conditions elevated levels of ApoB correlate to an increased risk of plaque formation in blood vessels. ApoB in conjunction with LDL receptor and other related proteins influences lipid homeostasis and disorders where lipid oxidation or accumulation is present. Monitoring ApoB and its concentration can be vital for assessing cardiovascular disease risk.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

See full target information APOB

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Apolipoprotein B抗体[HL1962] - BSA and Azide free

Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free (AB313868)

Indirect ELISA - Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free (AB313868)

Western blot - Anti-Apolipoprotein B antibody [HL1962] - BSA and Azide free (AB313868)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原