重组Anti-Apolipoprotein A I抗体[EPR20509-108] - BSA and Azide free (Capture) (ab281188)
![Sandwich ELISA - Anti-Apolipoprotein A I antibody [EPR20509-108] - BSA and Azide free (Capture) (ab281188)](https://www.abcam.cn/ps/products/281/ab281188/Images/ab281188-2-human-apoa1-elisa-kit-sandwich-elisa-standard-curve-8.jpg "Sandwich ELISA - Anti-Apolipoprotein A I antibody [EPR20509-108] - BSA and Azide free (Capture) (ab281188)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20509-108] to Apolipoprotein A I - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Human
概述
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产品名称
Anti-Apolipoprotein A I抗体[EPR20509-108] - BSA and Azide free (Capture)
参阅全部 Apolipoprotein A I 一抗 -
描述
兔单克隆抗体[EPR20509-108] to Apolipoprotein A I - BSA and Azide free (Capture) -
宿主
Rabbit -
经测试应用
适用于: Sandwich ELISAmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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常规说明
ab281188 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human APOA1 (Apolipoprotein A I). The recommended pair for sandwich ELISA is:
Capture: ab281188, Human APOA1 (Apolipoprotein A I) Capture Antibody (unconjugated)
Detector: ab281038, Human APOA1 (Apolipoprotein A I) Detector Antibody (unconjugated)
The reference range value is 150 - 10000 pg/mL.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20509-108 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- Streptavidin Conjugation Kit - Lightning-Link® (ab102921)
- Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
- Anti-Apolipoprotein A I antibody [EPR20509-23] - BSA and Azide free (Detector) (ab281038)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab281188于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR20509-23] to Apolipoprotein A I - BSA and Azide free (Detector) (ab281038).
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR20509-23] to Apolipoprotein A I - BSA and Azide free (Detector) (ab281038). |
靶标
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功能
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. -
组织特异性
Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. -
疾病相关
Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I.
Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed.
Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. -
序列相似性
Belongs to the apolipoprotein A1/A4/E family. -
翻译后修饰
Palmitoylated.
Phosphorylation sites are present in the extracelllular medium. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 335 Human
- Omim: 107680 Human
- SwissProt: P02647 Human
- Unigene: 93194 Human
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别名
- Apo-AI antibody
- ApoA I antibody
- ApoA-I antibody
see all
图片
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Sandwich ELISA - Anti-Apolipoprotein A I antibody [EPR20509-108] - BSA and Azide free (Capture) (ab281188)Example of Human APOA1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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![Anti-Apolipoprotein A I antibody [EPR20509-108] - BSA and Azide free (Capture) (ab281188)](https://www.abcam.cn/ps/products/281/ab281188/Images/ab281188-1-RecombinantAntibodyBenefitfigure.png)
Anti-Apolipoprotein A I antibody [EPR20509-108] - BSA and Azide free (Capture) (ab281188)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab281188 尚未被引用在任何文献中。
