Name: Anti-Apolipoprotein A I antibody [EP1368Y]
Brand: RabMAb
SKU: ab52945
Rating: 4 (9 reviews)

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Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1368Y] to Apolipoprotein A I
Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF, ELISA
Reacts with: Human, Recombinant fragment

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE

产品名称

Anti-Apolipoprotein A I抗体[EP1368Y]
参阅全部 Apolipoprotein A I 一抗
描述

兔单克隆抗体[EP1368Y] to Apolipoprotein A I
宿主

Rabbit
经测试应用

适用于: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF, ELISAmore details
种属反应性

与反应: Human, Recombinant fragment
免疫原

Synthetic peptide within Human Apolipoprotein A I aa 1-100 (N terminal). The exact sequence is proprietary.
阳性对照
- WB: Human liver and fetal lysates IHC-P: Human liver Tissue ICC/IF: HepG2 cells Flow Cyt (intra): HepG2 cells IP: HepG2 cell lysates, Human fetal liver tissue lysate
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EP1368Y
同种型

IgG
研究领域

The Abpromise guarantee

Abpromise™承诺保证使用ab52945于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Flow Cyt (Intra)		1/20.
WB	(5)	1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). For unpurified use at 1/20000 dilution.
IP		1/60.
IHC-P		1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
ICC/IF	(2)	1/100 - 1/250.
ELISA		Use at an assay dependent concentration. Antibody conc. = 0 - 1000 ng/ml

说明
Flow Cyt (Intra) 1/20.
WB 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). For unpurified use at 1/20000 dilution.
IP 1/60.
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
ICC/IF 1/100 - 1/250.
ELISA Use at an assay dependent concentration. Antibody conc. = 0 - 1000 ng/ml

功能

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
组织特异性

Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
疾病相关

Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I.
Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed.
Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
序列相似性

Belongs to the apolipoprotein A1/A4/E family.
翻译后修饰

Palmitoylated.
Phosphorylation sites are present in the extracelllular medium.
细胞定位

Secreted.
Target information above from: UniProt accession P02647 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 335 Human
- Omim: 107680 Human
- SwissProt: P02647 Human
- Unigene: 93194 Human
别名
- Apo-AI antibody
- ApoA I antibody
- ApoA-I antibody
- APOA1 antibody
- APOA1_HUMAN antibody
- Apolipoprotein A-I(1-242) antibody
- Apolipoprotein A1 antibody
- Apolipoprotein AI antibody
- Apolipoprotein of high density lipoprotein antibody
- ApolipoproteinAI antibody
- Brp14 antibody
- high density lipoprotein uptake antibody
- Ltw1 antibody
- Lvtw1 antibody
- MGC117399 antibody
- Sep1 antibody
- Sep2 antibody
see all

Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/1000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 31 kDa
Observed band size: 31 kDa
Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/1000 dilution (Purified) + Human liver lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 31 kDa
Observed band size: 31 kDa
Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/20000 dilution ((unpurified)) + fetal liver lysate at 10 µg

Secondary
goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 31 kDa
Observed band size: 31 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Apolipoprotein A I with purified ab52945 at 1/100 dilution (1.95 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Apolipoprotein A I with purified ab52945 at 1/250 dilution (0.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry (Intracellular) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Apolipoprotein A I with purified ab52945 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunoprecipitation - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

ab52945 (purified) at 1/20 dilution (1ug) immunoprecipitating Apolipoprotein A I in Human fetal liver lysates.
Lane 1: Human fetal liver lysates 10ug
Lane 2 (+): ab52945 & Human fetal liver lysates
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52945 in Human fetal liver lysates
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Indirect ELISA - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

ELISA analysis of Apolipoprotein A I recombinant protein at 1000 ng/mL with ab52945 at 1000~0ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

ab52945 (unpurified) at 1/100 dilution staining Apolipoprotein A I in human liver by Immunohistochemistry, Paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

ab52945 (unpurified) at 1/100 dilution staining Apolipoprotein A I in HEPG2 cells by Immunofluorescence.
Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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发表研究结果有使用 ab52945？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab52945 被引用在 42 文献中.

Kumar S et al. Quantifying PON1 on HDL with nanoparticle-gated electrokinetic membrane sensor for accurate cardiovascular risk assessment. Nat Commun 14:557 (2023). PubMed: 36732521
Wang C et al. Secreted endogenous macrosomes reduce Aβ burden and ameliorate Alzheimer's disease. Sci Adv 9:eade0293 (2023). PubMed: 37235655
Wang Y et al. Impact of apolipoprotein A1 on tumor immune microenvironment, clinical prognosis and genomic landscape in hepatocellular carcinoma. Precis Clin Med 6:pbad021 (2023). PubMed: 38025972
Solé M et al. Therapeutic effect of human ApoA-I-Milano variant in aged transgenic mouse model of Alzheimer's disease. Br J Pharmacol 180:1999-2017 (2023). PubMed: 36872299
Karvinen S et al. Extracellular vesicles and high-density lipoproteins: Exercise and oestrogen-responsive small RNA carriers. J Extracell Vesicles 12:e12308 (2023). PubMed: 36739598

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客户评价及客户问答

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1-10 of 13 Abreviews or Q&A

Application

Western blot

Sample

Bos taurus Tissue lysate - whole (Bovine reticulum and rumen lysates)

Gel Running Conditions

Reduced Denaturing (10%)

Loading amount

6 µg

Specification

Bovine reticulum and rumen lysates

Blocking step

Antibody diluent 2 as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 21°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 28 2022

Application

Western blot

Sample

Dog Tissue lysate - other (Cerebospinal fluid)

Gel Running Conditions

Reduced Denaturing (4–20% gel)

Loading amount

10 µg

Specification

Cerebospinal fluid

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 15 2022

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Mouse vascular smooth muscle cells)

Permeabilization

Yes - NP40

Specification

Mouse vascular smooth muscle cells

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 27 2021

Application

Western blot

Sample

Human Cell lysate - whole cell (U2OS)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

U2OS

Blocking step

Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 27 2021

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (U2OS)

Permeabilization

Yes - NP40

Specification

U2OS

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 27 2021

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Mouse vascular smooth muscle cells)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Specification

Mouse vascular smooth muscle cells

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 27 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Small Intestine)

Antigen retrieval step

Heat mediated

Permeabilization

Specification

Small Intestine

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Arnab Chattopadhyay

Verified customer

提交于 Oct 31 2018

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Mouse Tissue sections (Brain)

Permeabilization

Yes - 0.1 M PBS with 1% Triton X

Specification

Brain

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Ruma Raha-Chowdhury

Verified customer

提交于 Feb 05 2016

Application

Western blot

Sample

Mouse Tissue lysate - whole (Brain lysate)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Specification

Brain lysate

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Ruma Raha-Chowdhury

Verified customer

提交于 Jan 28 2016

Question

Inquiry: I would like to use a blocking peptide to check the specificity of my antibody by western-blot. The antibody I am using is EP1368Y (anti human Apoa-1). Could I obtaing this peptide used to produce the antibody? thank your very much,

Abcam community

Verified customer

Asked on Mar 26 2013

Answer

Thank you for your enquiry and interest in our products.

Currently, the blocking peptide for this particular antibody is not available commercially. However, if you wish to check the specificity I would advise to run a no-primary (only secondary antibody) control, a positive control (fetal liver lysate, human liver tissue and HEPG2 cells) and a negative control alongside with the samples.

You may find some useful information on how to find positive control at this site:

https://www.abcam.com/index.html?pageconfig=technicalfaqs#6

If you need any further assistance in the future, please do not hesitate to contact me.

Abcam Scientific Support

回复于 Mar 26 2013

1-10 of 13 Abreviews or Q&A

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

组织特异性

疾病相关

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (42)

客户评价及客户问答

Western blot abreview for Anti-Apolipoprotein A I antibody [EP1368Y]

Western blot abreview for Anti-Apolipoprotein A I antibody [EP1368Y]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Apolipoprotein A I antibody [EP1368Y]

Western blot abreview for Anti-Apolipoprotein A I antibody [EP1368Y]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Apolipoprotein A I antibody [EP1368Y]

Western blot abreview for Anti-Apolipoprotein A I antibody [EP1368Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Apolipoprotein A I antibody [EP1368Y]

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Apolipoprotein A I antibody [EP1368Y]

Western blot abreview for Anti-Apolipoprotein A I antibody [EP1368Y]