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Cardiovascular Lipids / Lipoproteins Lipoproteins/Apolipoproteins

Anti-Apolipoprotein A I抗体(ab7614)

  • Datasheet
  • SDS
Reviews (5)Q&A (8)References (15)

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Key features and details

  • Goat polyclonal to Apolipoprotein A I
  • Suitable for: ELISA, IP, IHC-P, Sandwich ELISA, WB
  • Reacts with: Mouse
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Apolipoprotein A I抗体
    参阅全部 Apolipoprotein A I 一抗
  • 描述

    山羊多克隆抗体to Apolipoprotein A I
  • 宿主

    Goat
  • 特异性

    Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. This antibody reacts with mouse apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins.
  • 经测试应用

    适用于: ELISA, IP, IHC-P, Sandwich ELISA, WBmore details
  • 种属反应性

    与反应: Mouse
  • 免疫原

    Full length native apoLipoprotein Type A-I (purified).

  • 常规说明

    This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.44% Sodium chloride, 4.77% Sodium borate, 0.15% EDTA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Signal Transduction
    • Metabolism
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相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human Apolipoprotein A I (ab50239)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7614于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P (1)
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration.
WB (3)
Use at an assay dependent concentration.
说明
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

靶标

  • 功能

    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 组织特异性

    Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
  • 疾病相关

    Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
    Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I.
    Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed.
    Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • 序列相似性

    Belongs to the apolipoprotein A1/A4/E family.
  • 翻译后修饰

    Palmitoylated.
    Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位

    Secreted.
  • Target information above from: UniProt accession P02647 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 11806 Mouse
    • SwissProt: Q00623 Mouse
    • 别名

      • Apo-AI antibody
      • ApoA I antibody
      • ApoA-I antibody
      • APOA1 antibody
      • APOA1_HUMAN antibody
      • Apolipoprotein A-I(1-242) antibody
      • Apolipoprotein A1 antibody
      • Apolipoprotein AI antibody
      • Apolipoprotein of high density lipoprotein antibody
      • ApolipoproteinAI antibody
      • Brp14 antibody
      • high density lipoprotein uptake antibody
      • Ltw1 antibody
      • Lvtw1 antibody
      • MGC117399 antibody
      • Sep1 antibody
      • Sep2 antibody
      see all

    实验方案

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (15)

    发表研究结果有使用 ab7614?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab7614 被引用在 15 文献中.

    • Yamada K  et al. Validation of serum apolipoprotein A1 in rabies virus-infected mice as a biomarker for the preclinical diagnosis of rabies. Microbiol Immunol 65:438-448 (2021). PubMed: 34270107
    • Song Z  et al. Comprehensive Proteomic Profiling of Urinary Exosomes and Identification of Potential Non-invasive Early Biomarkers of Alzheimer's Disease in 5XFAD Mouse Model. Front Genet 11:565479 (2020). PubMed: 33250918
    • Gluchowski NL  et al. Hepatocyte Deletion of Triglyceride-Synthesis Enzyme Acyl CoA: Diacylglycerol Acyltransferase 2 Reduces Steatosis Without Increasing Inflammation or Fibrosis in Mice. Hepatology 70:1972-1985 (2019). PubMed: 31081165
    • Belew MS  et al. PLAG1 and USF2 Co-regulate Expression of Musashi-2 in Human Hematopoietic Stem and Progenitor Cells. Stem Cell Reports 10:1384-1397 (2018). PubMed: 29641991
    • Cao J  et al. Protein markers of dysfunctional HDL in scavenger receptor class B type I deficient mice. J Transl Med 16:155 (2018). WB ; Mouse . PubMed: 29879989
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 13 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Apolipoprotein A I antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Liver)
    Permeabilization
    Yes - 0.25% Triton X-100
    Specification
    Liver
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Tomaz Martini

    Verified customer

    提交于 Aug 05 2022

    Western blot abreview for Anti-Apolipoprotein A I antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Loading amount
    10 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (4-20 %)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 02 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Apolipoprotein A I antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver, stomach, kidney, testes, lung, heart, etc)
    Specification
    Liver, stomach, kidney, testes, lung, heart, etc
    Fixative
    Formaldehyde
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Proteinase K
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 2%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Miss. Judit Marsillach

    Verified customer

    提交于 Jul 24 2009

    Western blot abreview for Anti-Apolipoprotein A I antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Loading amount
    50 µg
    Specification
    liver
    Gel Running Conditions
    Reduced Denaturing (9%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Hua Jiang

    Verified customer

    提交于 Jan 02 2009

    Western blot abreview for Anti-Apolipoprotein A I antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Purified protein (purified protein (BIODESIGN))
    Loading amount
    0.5 µg
    Specification
    purified protein (BIODESIGN)
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    5%mill+1%BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 11 2008

    Question

    I want to know if you have mouse APOA1, APOB and APOCIII ELISAs? If you have, please give me a quotation of two for each of them.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 10 2012

    Answer

    Thank you for contacting us. We currently only have a mouse ELISA kit for Apolipoprotein CIII: ab108811 However, we do have antibodies to each of your targets of interest that can be used in ELISA on mouse samples: Apolipoprotein A I: ab7614 or ab20453 Apolipoprotein B: ab117317 Apolipoprotein CIII: ab55984 If you could let me know which products you are interested in and what quantities, I would be happy to create a proforma invoice for you.

    Read More

    Abcam Scientific Support

    回复于 Jan 10 2012

    Question

    How was this antibody tested in ELISA? Was it tested on mouse serum? What dilutions of serum were used? I am not seeing a dose-dependent signal.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 24 2006

    Answer

    Thank you for your enquiry. This product was tested using a direct ELISA using purified mouse Apo A-1. It was not tested using different concentrations of mouse serum. I am sorry you are experiencing problems with this antibody. Below please find a link to our troubleshooting questionnaire. This will give us further information regarding the samples you tested and the protocol you used. Once you have submitted the form to us, we will look at the protocol you used and see if there are any suggestions we can make to improve your results. The questionnaire can be found at: https://www.abcam.com/index.html?section=elisa&pageconfig=technical&intAbID=7614&mode=questionaire I look forward to receiving your reply.

    Read More

    Abcam Scientific Support

    回复于 Oct 24 2006

    Question

    BATCH NUMBER 57140 ORDER NUMBER 58914 DESCRIPTION OF THE PROBLEM The antibody did not stain specific protein in arterial tissue (immunhistochemistry of formalin fixed tissue), in peripheral blood mononuclear cells (cytospin of native cells) nor in plasma (western blot). The only detectable signal in the tissue was in leukocytes (presumably false positive peroxidase signal). Simultaneously we used your antibody against ApoB100 (ab7616) which worked perfectly fine, both in tissue and in the western blot. SAMPLE human arterial tissue, liver and kidney. human plasma human peripheral blood mononuclear cells (PBMC) PRIMARY ANTIBODY tissue: ab7614; 10?g/ml (1:100; 1: 50 and 1:200 were also tested) in Antibody diluent (DAKO S2022); 1h RT Western blot: ab7614; 1?g/ml 5% fat free milk/TBS, 1h RT wash: tissue 2x in PBS; Western blot 3x in TBS. SECONDARY ANTIBODY tissue: Medite, NCL-RTU-Qu. Novostain Universal Quick kit, 30min RT Western blot: peroxidase conjugated rabbit anti goat DAKO (P0449) 0.1?g/ml in 5% fat-free milk/TBS; 1h RT wash: tissue 2x in PBS; Western blot 3x in TBS. DETECTION METHOD tissue: DAB (DAKO K3468) western blot: super signal West Pico kit (Pierce 1856135 + 1856136) POSITIVE AND NEGATIVE CONTROLS USED Plasma contains known amount of ApoA1 (1.5mg/ml) as a positive control tissue we used liver. we did not use purified protein nor deficiency plasma. ANTIBODY STORAGE CONDITIONS after arrival, 5 aliquots (20?l) were pipeted and stored frozen at -20? after thawing one aliquot for usage, the ab stored at 4? and not refrozen. the interval between antibody arrival and aliquotation was 5 days and the first aliquot was immediately. FIXATION OF SAMPLE tissues were fixed in 4% phosphate buffered formalin plasma and PBMC were used unfixed ANTIGEN RETRIEVAL used only for formalin fixed tissues: proteinase K was used according to the manufacturer's description (DAKO S3020) alternatively we tried citrate buffer in heat pressure chamber but this resulted in high background staining PERMEABILIZATION STEP The only procedure were permeabilization was used was in cytospin preps of PBMCs. We used 0.1% saponin in PBS-FCS. BLOCKING CONDITIONS for tissue: Medite, normal horse serum, NCL-H-serum, ready for use for western blot: 5% fat free milk in TBS (pH 7.4) for cytospin: 1% BSA-1%FCS in PBS (pH 7.4) HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? >10 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? primary antibody dilution alternative detection approaches such as western blot. different presumably positive tissues. more 300 arterial specimens were stained and were negative ! we ordered a second antibody lot ! ADDITIONAL NOTES Since we are very interested in the reliable detection of apolipoprotein A1 in arterial tissues we are looking forward to your suggestions.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 23 2005

    Answer

    Thank you for your enquiry and for taking your time to fill in the on-line Questionnaire. We are very sorry to hear that you are having problem with this antibody. We have searched our database and found that this is a popular selling product and your feedback is the first we have received about it not working. We would like to emphasize the fact that this antibody has been tested for cross-reaction only with mouse but other species have not been characterized yet. Therefore, unfortunately, we do not know if it recognizes human Apolipoprotein A I. We would suggest using mouse samples as positive control along with the human samples to make sure that the detection system works properly. Since you are using this antibody on human samples which has not been tested yet, we would appreciate any feedback information about your results which we could share with our present and future customers. Please let us know how you get on, and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards. We hope this information will be useful for you.

    Read More

    Abcam Scientific Support

    回复于 Feb 28 2005

    Question

    We purchased the ab7614 goat polyclonal antibody against apolipoprotein A-I to develop an ELISA for measuring apo A-I in mouse plasma samples, do you have experience with this application and could you advise me on how to setup this assay?

    Read More

    Abcam community

    Verified customer

    Asked on May 12 2004

    Answer

    Thank you for your enquiry. We would suggest performing a regular sandwich ELISA protocol.If you need anything further or any help then please let us know.

    Read More

    Abcam Scientific Support

    回复于 May 12 2004

    Question

    I have a customer who wishes to know what species the full-length immunogen protein is from. Mouse or human?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 25 2004

    Answer

    Thank you for your enquiry. The immunogen is from mouse that's why the antibody recognizes mouse Apolipoprotein A I.

    Read More

    Abcam Scientific Support

    回复于 Mar 26 2004

    1-10 of 13 Abreviews or Q&A

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