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Cardiovascular Lipids / Lipoproteins Lipoproteins/Apolipoproteins

Anti-Apolipoprotein A I抗体(ab7613)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (40)

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Key features and details

  • Goat polyclonal to Apolipoprotein A I
  • Suitable for: IP, WB, ELISA, IHC-P
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Apolipoprotein A I抗体
    参阅全部 Apolipoprotein A I 一抗
  • 描述

    山羊多克隆抗体to Apolipoprotein A I
  • 宿主

    Goat
  • 特异性

    Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross-reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

  • 经测试应用

    适用于: IP, WB, ELISA, IHC-Pmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Full length native apoLipoprotein Type A-I (purified).

  • 常规说明

    This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 8.00
    Preservative: 0.01% Sodium azide
    Constituents: 4.77% Sodium borate, 0.44% Sodium chloride, 0.15% EDTA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Goat Anti-Apolipoprotein A-I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Apolipoproteins
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Kits/ Lysates/ Other
    • Kits
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    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
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    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Lipoprotein ELISA Kits

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human Apolipoprotein A I (ab50239)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7613于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
1/100.
WB (3)
1/1000 - 1/2000.
ELISA
1/10000 - 1/20000.

Can be paired with ab20784 for sandwich ELISA.

IHC-P
1/50 - 1/200.
说明
IP
1/100.
WB
1/1000 - 1/2000.
ELISA
1/10000 - 1/20000.

Can be paired with ab20784 for sandwich ELISA.

IHC-P
1/50 - 1/200.

靶标

  • 功能

    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 组织特异性

    Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
  • 疾病相关

    Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
    Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I.
    Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed.
    Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • 序列相似性

    Belongs to the apolipoprotein A1/A4/E family.
  • 翻译后修饰

    Palmitoylated.
    Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位

    Secreted.
  • Target information above from: UniProt accession P02647 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 335 Human
    • Omim: 107680 Human
    • SwissProt: P02647 Human
    • Unigene: 93194 Human
    • 别名

      • Apo-AI antibody
      • ApoA I antibody
      • ApoA-I antibody
      • APOA1 antibody
      • APOA1_HUMAN antibody
      • Apolipoprotein A-I(1-242) antibody
      • Apolipoprotein A1 antibody
      • Apolipoprotein AI antibody
      • Apolipoprotein of high density lipoprotein antibody
      • ApolipoproteinAI antibody
      • Brp14 antibody
      • high density lipoprotein uptake antibody
      • Ltw1 antibody
      • Lvtw1 antibody
      • MGC117399 antibody
      • Sep1 antibody
      • Sep2 antibody
      see all

    实验方案

    • Immunoprecipitation protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (40)

    发表研究结果有使用 ab7613?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab7613 被引用在 40 文献中.

    • Yoshinaga T  et al. Giant Hepatomegaly with Spleno-testicular Enlargement in a Patient with Apolipoprotein A-I Amyloidosis: An Uncommon Type of Amyloidosis in Japan. Intern Med 60:575-581 (2021). PubMed: 32999221
    • Holzwirth E  et al. Anti-inflammatory HDL effects are impaired in atrial fibrillation. Heart Vessels N/A:N/A (2021). PubMed: 34459957
    • Tougan T  et al. The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity. Malar J 19:155 (2020). PubMed: 32295584
    • Mead B  et al. TNFa-Mediated Priming of Mesenchymal Stem Cells Enhances Their Neuroprotective Effect on Retinal Ganglion Cells. Invest Ophthalmol Vis Sci 61:6 (2020). PubMed: 32031578
    • Dong L  et al. Comprehensive evaluation of methods for small extracellular vesicles separation from human plasma, urine and cell culture medium. J Extracell Vesicles 10:e12044 (2020). PubMed: 33489012
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-Apolipoprotein A I antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Tissue lysate - other (Intestinal biopsy)
    Gel Running Conditions
    Reduced Denaturing (8–16% Mini-PROTEAN)
    Loading amount
    10 µg
    Specification
    Intestinal biopsy
    Blocking step
    PBS NFDM as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Floris C Droees

    Verified customer

    提交于 Jan 23 2020

    Western blot abreview for Anti-Apolipoprotein A I antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Purified protein (purified apoAI (BIODESIGN))
    Loading amount
    0.5 µg
    Specification
    purified apoAI (BIODESIGN)
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    5%milk+1%BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 23 2008

    Western blot abreview for Anti-Apolipoprotein A I antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Purified protein (human plasma)
    Loading amount
    50 µg
    Specification
    human plasma
    Treatment
    Plama was separated into different bands by agarose electrophoresis, and alphaHDL was cut and protein was extracted for 2nd dimensional SDS-PAGE
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. xuefei li

    Verified customer

    提交于 Sep 19 2007

    Question

    Do you know if ab7613 reacts with plasminogen as well as apolipoprotein A? Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Oct 12 2012

    Answer

    Thank you for contacting Abcam.

    We havenot tested ab7613specifically against plasminogen, and cross-reaction with plasminogen is not detectable by our QC Testing.

    Please let me know if you have any further.

    Read More

    Abcam Scientific Support

    回复于 Oct 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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