重组Anti-APOBEC3G/A3G抗体[EPR25404-59] - BSA and Azide free (ab302927)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25404-59] to APOBEC3G/A3G - BSA and Azide free
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-APOBEC3G/A3G抗体[EPR25404-59] - BSA and Azide free
参阅全部 APOBEC3G/A3G 一抗 -
描述
兔单克隆抗体[EPR25404-59] to APOBEC3G/A3G - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IPmore details
不适用于: Flow Cyt (Intra) or ICC/IF -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: H9 whole cell lysate. 293T transfected with human APOBEC3G expression vector containing a myc-His-tag®. Human tonsil tissue lysate. IHC-P: Human tonsil tissue. Human breast cancer tissue. Wild-type H9 cell pellet. IP: H9 whole cell lysate.
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常规说明
ab302927 is the carrier-free version of ab302926.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.20
Constituent: 100% PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR25404-59 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab302927于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 46 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 46 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. |
靶标
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功能
DNA deaminase (cytidine deaminase) that mediates a form of innate resistance to retroviral infections (at least to HIV-1 infection) by triggering G-to-A hypermutation in the newly synthesized viral DNA. The replacements C-to-U in the minus strand DNA of HIV-1 during reverse transcription, leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. Modification of both DNA strands is not excluded. This antiviral activity is neutralized by the virion infectivity factor (VIF), that prevents the incorporation of APOBEC3G into progeny HIV-1 virions by both inhibiting its translation and/or by inducing its ubiquitination and subsequent degradation by the 26S proteasome. APOBEC3G binds a variety of RNAs, but does not display detectable APOB, NF1 and NAT1 mRNA editing. -
组织特异性
Expressed in spleen, testes, ovary and peripheral blood leukocytes and CD4+ lymphocytes. Also expressed in non-permissive peripheral blood mononuclear cells, and several tumor cell lines; no expression detected in permissive lymphoid and non-lymphoid cell lines. -
序列相似性
Belongs to the cytidine and deoxycytidylate deaminase family. -
翻译后修饰
Ubiquitinated in the presence of HIV-1 VIF. Association with VIF targets the protein for proteolysis by the ubiquitin-dependent proteasome pathway. -
细胞定位
Cytoplasm. Nucleus. Mainly cytoplasmic. Small amount are found in the nucleus. During HIV-1 infection, virion-encapsidated in absence of HIV-1 VIF. - Information by UniProt
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数据库链接
- Entrez Gene: 200316 Human
- Entrez Gene: 60489 Human
- Omim: 607113 Human
- SwissProt: Q9HC16 Human
- Unigene: 659809 Human
- Unigene: 660143 Human
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别名
- A3G antibody
- ABC3G_HUMAN antibody
- APOBEC related cytidine deaminase antibody
see all
图片
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All lanes : Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926) at 1/1000 dilution
Lane 1 : H9 (human lymphoma T lymphocyte), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?This data was developed using ab302926, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Jurkat (PMID: 14527406)
Exposure time: 180 seconds
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All lanes : Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926) at 1/1000 dilution
Lane 1 : 293T transfected with human APOBEC3G expression vector containi a myc-His-tag®, whole cell lysate
Lane 2 : 293T transfected with human APOBEC3A expression vector containi a myc-His-tag®, whole cell lysate, 20
Lane 3 : 293T transfected with human APOBEC3B expression vector containi a myc-His-tag®, whole cell lysate, 20
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 46 kDaThis data was developed using ab302926, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with human APOBEC3A or APOBEC3B
Exposure time: 180 seconds
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All lanes : Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926) at 1/1000 dilution
Lane 1 : Human tonsil tissue lysate
Lane 2 : Human heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 46 kDaThis data was developed using ab302926, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 180 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC3G/A3G antibody [EPR25404-59] - BSA and Azide free (ab302927)
This data was developed using AB302926, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling APOBEC3G/A3G with AB302926 at 1/2000 (0.255 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on immune cells in human tonsil.
The section was incubated with ab302926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC3G/A3G antibody [EPR25404-59] - BSA and Azide free (ab302927)
This data was developed using AB302926, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling APOBEC3G/A3G with AB302926 at 1/2000 (0.255 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on immune cells in human breast cancer.
The section was incubated with ab302926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC3G/A3G antibody [EPR25404-59] - BSA and Azide free (ab302927)
This data was developed using AB302926, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded wild-type H9 cell pellet labeling APOBEC3G/A3G with AB302926 at 1/2000 (0.255 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on wild-type H9 cell pellet (Panel A); No staining on wild-type Jurkat cell pellet (Panel B); No staining on wild-type 293T cell pellet (Panel C). The section was incubated with ab302926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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This data was developed using AB302926, the same antibody clone in a different buffer formulation.
APOBEC3G/A3G was immunoprecipitated from 0.35 mg H9 (human lymphoma T lymphocyte) whole cell lysate 20 ug with AB302926 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using AB302926 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: H9 whole cell lysate 20 ug
Lane 2: AB302926 IP in H9 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302926 in H9 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
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