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重组RabMAb

重组Anti-APOA4/Apo-AIV抗体[EPR2895] (ab108200)

Reviews (1) Submit a question References (1)
Western blot - Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)
  • Immunoprecipitation - Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)
  • Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2895] to APOA4/Apo-AIV
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-APOA4/Apo-AIV抗体[EPR2895]
    参阅全部 APOA4/Apo-AIV 一抗

  • 描述

    兔单克隆抗体[EPR2895] to APOA4/Apo-AIV

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Human plasma

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab108200于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 45 kDa).
IP
1/10 - 1/100.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 45 kDa).
IP
1/10 - 1/100.
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

靶标

  • 相关性

    APOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties

  • 细胞定位

    Secreted

  • 数据库链接

    • 别名

      • Apo AIV antibody
      • APOA 4 antibody
      • ApoA IV antibody
      • APOA4 antibody
      • Apolipoprotein A IV antibody
      • Apolipoprotein A4 antibody
      • MGC142154 antibody
      • MGC142156 antibody
      see all

    图片

    • Western blot - Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)
      Western blot - Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)
      Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200) at 1/1000 dilution + Human plasma at 10 µg

      Predicted band size: 45 kDa

    • Immunoprecipitation - Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)
      Immunoprecipitation - Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)

      Western blot analysis of APOA4/Apo-AIV in immunoprecipitation pellets from Human plasma (Lane 1) or 1XPBS (negative control) (Lane 2) using ab108200 at 1/10 dilution for IP and at 1/1000 dilution for WB detection.

    • Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)
      Anti-APOA4/Apo-AIV antibody [EPR2895] (ab108200)

    文献 (1)

    发表研究结果有使用 ab108200？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab108200 被引用在 1 文献中.

    • Cubedo J  et al. ApoL1 levels in high density lipoprotein and cardiovascular event presentation in patients with familial hypercholesterolemia. J Lipid Res 57:1059-73 (2016). PubMed: 27112635

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Western blot abreview for Anti-APOA4 antibody [EPR2895]

     Poor
    Abreviews
    Application
    Western blot
    Sample
    Human Serum (human plasma)
    Loading amount
    0.5 µg
    Specification
    human plasma
    Gel Running Conditions
    Reduced Denaturing (9%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Hua Jiang

    Verified customer

    提交于 Aug 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com