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AB242896

重组Anti-APOA4/Apo-AIV抗体[EPR19465-41] - BSA and Azide free (Detector)

Anti-APOA4/Apo-AIV antibody [EPR19465-41] - BSA and Azide free (Detector)

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(1 Publication)

Rabbit Recombinant Monoclonal APOA4/Apo-AIV antibody. Carrier free. Suitable for sELISA and reacts with Human samples. Cited in 1 publication.

查看别名

Apolipoprotein A-IV, Apo-AIV, ApoA-IV, Apolipoprotein A4, APOA4

1 Images
Sandwich ELISA - Anti-APOA4/Apo-AIV antibody [EPR19465-41] - BSA and Azide free (Detector) (AB242896)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-APOA4/Apo-AIV antibody [EPR19465-41] - BSA and Azide free (Detector) (AB242896)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab214567).

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19465-41

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

产品详情

ab242896 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Apolipoprotein A-IV. The recommended pair for sandwich ELISA is:
Capture: ab242651, Human Apolipoprotein A-IV Capture Antibody (unconjugated)
Detector: ab242896, Human Apolipoprotein A-IV Detector Antibody (unconjugated)
The reference range value is 250 - 16000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

APOA4 also known as Apo-AIV or apolipoprotein A4 is a protein mainly expressed in the small intestine and liver. It has a molecular mass of approximately 46 kDa. This protein plays a significant role in lipid metabolism by facilitating the transport of lipids in the bloodstream. APOA4 exists in the plasma and associates predominately with high-density lipoproteins (HDL) and to a lesser extent with chylomicrons.
Biological function summary

Apolipoprotein A4 acts as an important player in the regulation of lipid homeostasis and satiety. This protein is not part of a complex but enhances the efficiency of lipid absorption and transport. APOA4 reacts with enzymes like lecithin-cholesterol acyltransferase which is essential in the maturation of HDL particles. This interaction supports cholesterol efflux and assists in maintaining cholesterol balance within the body.

Pathways

APOA4 is heavily involved in the reverse cholesterol transport pathway which is important for removing excess cholesterol from cells and tissues taking it back to the liver for excretion. Within this pathway APOA4 interacts with key proteins like apolipoprotein A1 and lecithin-cholesterol acyltransferase to aid in this lipid transport mechanism. It also participates in a related lipid homeostasis pathway enhancing lipid absorption efficiency.

Research links APOA4 to atherosclerosis and obesity. Altered expression or function of apolipoprotein A4 often correlates with increased cardiovascular disease risk due to its role in cholesterol transport. It is associated with apolipoprotein A1 as both of these proteins significantly impact lipid profiles related to cardiovascular health. The ability of APOA4 to influence satiety also connects it to obesity suggesting a role in energy balance regulation.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
See full target information APOA4

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational gastroenterology 14:e00639 PubMed37753949

2023

Serological Investigation of Persistent Villous Atrophy in Celiac Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Changlin Gong,Claudia Saborit,Xin Long,Ao Wang,Beishi Zheng,Howard Chung,Suzanne K Lewis,Suneeta Krishnareddy,Govind Bhagat,Peter H R Green,Xiao-Fei Kong
View all publications

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