重组Anti-Apo-D抗体[EPR2916] (ab108191)
![Western blot - Anti-Apo-D antibody [EPR2916] (ab108191)](https://www.abcam.cn/ps/products/108/ab108191/Images/ab108191-254375-anti-apo-d-antibody-epr2916-western-blot.jpg "Western blot - Anti-Apo-D antibody [EPR2916] (ab108191)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2916] to Apo-D
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Apo-D抗体[EPR2916]
参阅全部 Apo-D 一抗 -
描述
兔单克隆抗体[EPR2916] to Apo-D -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human plasma cell lysate IHC-P: Human kidney or liver tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2916 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108191于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts. -
组织特异性
Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears. -
序列相似性
Belongs to the calycin superfamily. Lipocalin family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 347 Human
- Omim: 107740 Human
- SwissProt: P05090 Human
- Unigene: 522555 Human
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别名
- APO D antibody
- Apo-D antibody
- ApoD antibody
see all
图片
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Western blot - Anti-Apo-D antibody [EPR2916] (ab108191)Anti-Apo-D antibody [EPR2916] (ab108191) at 1/2000 dilution (purified) + human plasma at 10 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 21 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)](https://www.abcam.cn/ps/products/108/ab108191/Images/ab108191-253426-anti-apo-d-antibody-epr2916-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)Immunohistochemical staining of paraffin embedded human liver with purified ab108191 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Western blot - Anti-Apo-D antibody [EPR2916] (ab108191)](https://www.abcam.cn/ps/products/108/ab108191/Images/ab108191-120682-anti-apo-d-antibody-epr2916-western-blot.jpg)
Western blot - Anti-Apo-D antibody [EPR2916] (ab108191)Anti-Apo-D antibody [EPR2916] (ab108191) at 1/1000 dilution (unpurified) + Human plasma cell lysate at 10 µg
Predicted band size: 21 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)](https://www.abcam.cn/ps/products/108/ab108191/Images/ab108191-120681-anti-apo-d-antibody-epr2916-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)Immunohistochemical staining of Apo-D in paraffin embedded human kidney tissue, using unpurified ab108191 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)](https://www.abcam.cn/ps/products/108/ab108191/Images/ab108191-120680-anti-apo-d-antibody-epr2916-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)Immunohistochemical staining of Apo-D in paraffin embedded Human liver tissue, using unpurified ab108191 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Anti-Apo-D antibody [EPR2916] (ab108191)](https://www.abcam.cn/ps/products/108/ab108191/Images/ab108191-7-benefits-of-recombinant-antibodies.png)
Anti-Apo-D antibody [EPR2916] (ab108191)
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab108191 被引用在 5 文献中.
- Cao M et al. Differential antigen expression between human apocrine sweat glands and eccrine sweat glands. Eur J Histochem 67:N/A (2023). PubMed: 36546419
- Wang Y et al. Single-cell analysis of pancreatic ductal adenocarcinoma identifies a novel fibroblast subtype associated with poor prognosis but better immunotherapy response. Cell Discov 7:36 (2021). PubMed: 34035226
- Qiu F et al. Development and Verification of an Immune-Based Gene Signature for Risk Stratification and Immunotherapeutic Efficacy Assessment in Gastric Cancer. Dis Markers 2021:4251763 (2021). PubMed: 34804261
- Toyoda Y et al. Clinical and Molecular Evidence of ABCC11 Protein Expression in Axillary Apocrine Glands of Patients with Axillary Osmidrosis. Int J Mol Sci 18:N/A (2017). PubMed: 28212277
- Li H et al. Apolipoprotein D modulates amyloid pathology in APP/PS1 Alzheimer's disease mice. Neurobiol Aging 36:1820-33 (2015). PubMed: 25784209