AB290679

重组Anti-Apo-D抗体[EPR24517-79] - BSA and Azide free (Detector)

Anti-Apo-D antibody [EPR24517-79] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Apo-D antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

Apolipoprotein D, Apo-D, ApoD, APOD

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Sandwich ELISA - Anti-Apo-D antibody [EPR24517-79] - BSA and Azide free (Detector) (AB290679)

sELISA

Supplier Data

Sandwich ELISA - Anti-Apo-D antibody [EPR24517-79] - BSA and Azide free (Detector) (AB290679)

Sandwich ELISA of ab290704 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR24517-79

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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产品详情

ab290679 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Apo-D. The recommended pair for sandwich ELISA is:
Capture: ab290678 , Human Apo-D Capture Antibody (unconjugated)
Detector: ab290679 , Human Apo-D Detector Antibody (unconjugated)
The reference range value is 19.53 - 2500 pg/mL.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Apo-D also known as apolipoprotein D is a glycoprotein with an approximate mass of 29 kilodaltons. As an apolipoprotein Apo-D is part of the lipocalin family which participates in the transport of small hydrophobic molecules. It is widely expressed in human tissues particularly in the brain liver and adrenal glands. The expression of Apo-D in these tissues suggests important roles in diverse physiological processes and lipid metabolism. Scientists often study Apo-D to understand its involvement in lipid transport and its biological activities.

Biological function summary

Apo-D interacts with various molecules affecting lipid and cholesterol transport and metabolism. Apo-D operates as part of a protein complex and binds ligands such as progesterone and arachidonic acid. These interactions suggest it might modulate their availability and activity in biological systems. Furthermore the role of Apo-D in protecting cells against oxidative stress emerges as an important mechanism of action contributing to its protective properties in neural and other tissues.

Pathways

Apo-D plays significant roles by influencing the HDL (high-density lipoprotein) metabolism pathway and the arachidonic acid pathway. Within the HDL pathway Apo-D associates with other apolipoproteins like apoA-I to regulate cholesterol transport and anti-inflammatory responses. Its involvement in the arachidonic acid pathway suggests a possible regulatory effect on inflammation and cellular stress responses highlighting the complex interaction of Apo-D with other proteins like cyclooxygenases which are key players in inflammation.

Researchers have linked Apo-D to conditions such as Alzheimer's disease and schizophrenia. In Alzheimer's disease its altered expression in the brain suggests a protective response or involvement in disease progression possibly through interaction with amyloid precursor proteins or influencing cholesterol metabolism. In schizophrenia abnormal levels of Apo-D in cerebrospinal fluid mark potential disturbance in lipid metabolism making it a protein of interest in the disease's complex pathophysiology. Understanding these connections provides insight into therapeutic strategies that target the biological functions of Apo-D.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.

See full target information Apolipoprotein D

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Abcam Product Promise

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重组Anti-Apo-D抗体[EPR24517-79] - BSA and Azide free (Detector)

Anti-Apo-D antibody [EPR24517-79] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-Apo-D antibody [EPR24517-79] - BSA and Azide free (Detector) (AB290679)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

反应性数据

产品详情

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

Abcam Product Promise

重组Anti-Apo-D抗体[EPR24517-79] - BSA and Azide free (Detector)

Anti-Apo-D antibody [EPR24517-79] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-Apo-D antibody [EPR24517-79] - BSA and Azide free (Detector) (AB290679)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

搭配使用产品

Assay Kits

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Assay Kits

Primary Antibodies

Primary Antibodies

反应性数据

产品详情

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

Abcam Product Promise