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重组

重组Anti-APLP1抗体[AP1] - BSA and Azide free (ab273469)

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Western blot - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
  • Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [AP1] to APLP1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-APLP1抗体[AP1] - BSA and Azide free
    参阅全部 APLP1 一抗

  • 描述

    小鼠单克隆抗体[AP1] to APLP1 - BSA and Azide free

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: IHC-Fr

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Mouse brain cortex tissue lysate. IHC-P: Human, mouse and rat cerebrum tissue; Human cerebrum Alzheimer's disease tissue.

  • 常规说明

    ab273469 is the carrier-free version of ab273448.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

性能

相关产品

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab273469于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Detects a band of approximately 92 kDa (predicted molecular weight: 72 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
说明
WB
Use at an assay dependent concentration. Detects a band of approximately 92 kDa (predicted molecular weight: 72 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
应用说明
Is unsuitable for IHC-Fr.

靶标

  • 功能

    May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I.
    The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis.

  • 组织特异性

    Expressed in the cerebral cortex where it is localized to the postsynaptic density (PSD).

  • 序列相似性

    Belongs to the APP family.

  • 结构域

    The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain. However, additional amino acids either N- or C-terminal to the NPXY motif are often required for complete interaction. The NPXY site is also involved in clathrin-mediated endocytosis.

  • 翻译后修饰

    Proteolytically cleaved by caspases during neuronal apoptosis. Cleaved, in vitro, at Asp-620 by caspase-3.
    N- and O-glycosylated. O-glycosylation with core 1 or possibly core 8 glycans. Glycosylation on Ser-227 is the preferred site to Thr-228.

  • 细胞定位

    Cell membrane and Cytoplasm. C-terminally processed in the Golgi complex.
  • Information by UniProt

  • 数据库链接

    • 别名

      • AMYLOID BETA A4 PRECURSOR-LIKE PROTEIN 1 antibody
      • AMYLOID PRECURSOR-LIKE PROTEIN antibody
      • Amyloid-like protein 1 precursor antibody
      • APLP 1 antibody
      • APLP antibody
      • APLP-1 antibody
      • Aplp1 antibody
      • APLP1_HUMAN antibody
      • C30 antibody
      see all

    图片

    • Western blot - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
      Western blot - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
      Anti-APLP1 antibody [AP1] (ab273448) at 1/1000 dilution + Mouse brain cortex tissue lysate at 20 µg

      Secondary
      Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Predicted band size: 72 kDa


      Exposure time: 37 seconds


      This data was developed using ab273448, the same antibody clone in a different buffer formulation.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)

      This data was developed using ab273448, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling APLP1 with ab273448 at 1/500 dilution followed by a ready to use secondary. Positive staining on human cerebrum is observed. The section was incubated with ab273448 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use secondary.
      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)

      This data was developed using ab273448, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human cerebrum with Alzheimer's disease tissue labeling APLP1 with ab273448 at 1/500 dilution followed by a ready to use secondary. Positive staining on human cerebrum with Alzheimer's disease is observed. The section was incubated with ab273448 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use secondary.
      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)

      This data was developed using ab273448, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling APLP1 with ab273448 at 1/500 dilution followed by a ready to use secondary. Positive staining on mouse cerebrum is observed. The section was incubated with ab273448 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use secondary.
      Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)

      This data was developed using ab273448, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling APLP1 with ab273448 at 1/500 dilution followed by a ready to use secondary. Positive staining on rat cerebrum is observed. The section was incubated with ab273448 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use secondary.
      Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    • Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
      Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab273469？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab273469 尚未被引用在任何文献中。

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