为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Base Excision Repair
使用敲除细胞株进行验证

Anti-APE1抗体[13B8E5C2] (ab194)

  • Datasheet
Reviews (10)Q&A (4)References (42)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-APE1 antibody [13B8E5C2] - ChIP Grade (ab194)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody [13B8E5C2] - ChIP Grade (ab194)
  • Western blot - Anti-APE1 antibody [13B8E5C2] - ChIP Grade (ab194)

Key features and details

  • Mouse monoclonal [13B8E5C2] to APE1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

你可能会感兴趣

蛋白
Product image
Recombinant Human APE1 protein (ab103260)
一抗
Product image
Anti-POLG antibody [EPR7296] (ab128899)
一抗
Product image
Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

查看更多相关产品

概述

  • 产品名称

    Anti-APE1抗体[13B8E5C2]
    参阅全部 APE1 一抗
  • 描述

    小鼠单克隆抗体[13B8E5C2] to APE1
  • 宿主

    Mouse
  • 特异性

    This antibody is specific to the human APE/ref-1 protein.
  • 经测试应用

    适用于: WB, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Full length native protein (purified) (Human).

  • 阳性对照

    • WB: HepG2, HEK293 and wild-type HAP1 cell lysates. IHC-P: Human normal placenta tissue.
  • 常规说明

    APE appears to form a unique link between the DNA base excision pathway, oxidative signalling, trancription regulation, cancer and cell-cycle control.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • 克隆

    单克隆
  • 克隆编号

    13B8E5C2
  • 骨髓瘤

    unknown
  • 同种型

    IgG2b
  • 轻链类型

    unknown
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Base Excision Repair
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators

相关产品

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant Human APE1 protein (ab103260)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab194于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (4)
1/2000. Predicted molecular weight: 35.6 kDa.
IHC-P
1/5000.
说明
WB
1/2000. Predicted molecular weight: 35.6 kDa.
IHC-P
1/5000.

靶标

  • 功能

    Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA.
  • 序列相似性

    Belongs to the DNA repair enzymes AP/ExoA family.
  • 结构域

    The N-terminus contains the redox activity while the C-terminus exerts the DNA AP-endodeoxyribonuclease activity; both function are independent in their actions. An unconventional mitochondrial targeting sequence (MTS) is harbored within the C-terminus, that appears to be masked by the N-terminal sequence containing the nuclear localization signal (NLS), that probably blocks the interaction between the MTS and Tom proteins.
  • 翻译后修饰

    Phosphorylated. Phosphorylation by kinase PKC or casein kinase CK2 results in enhanced redox activity that stimulates binding of the FOS/JUN AP-1 complex to its cognate binding site. AP-endodeoxyribonuclease activity is not affected by CK2-mediated phosphorylation. Phosphorylation of Thr-233 by CDK5 reduces AP-endodeoxyribonuclease activity resulting in accumulation of DNA damage and contributing to neuronal death.
    Acetylated on Lys-6 and Lys-7. Acetylation is increased by the transcriptional coactivator EP300 acetyltransferase, genotoxic agents like H(2)O(2) and methyl methanesulfonate (MMS). Acetylation increases its binding affinity to the negative calcium response element (nCaRE) DNA promoter. The acetylated form induces a stronger binding of YBX1 to the Y-box sequence in the MDR1 promoter than the unacetylated form. Deacetylated on lysines. Lys-6 and Lys-7 are deacetylated by SIRT1.
    Cleaved at Lys-31 by granzyme A to create the mitochondrial form; leading in reduction of binding to DNA, AP endodeoxynuclease activity, redox activation of transcription factors and to enhanced cell death. Cleaved by granzyme K; leading to intracellular ROS accumulation and enhanced cell death after oxidative stress.
    Cys-65 and Cys-93 are nitrosylated in response to nitric oxide (NO) and lead to the exposure of the nuclear export signal (NES).
    Ubiquitinated by MDM2; leading to translocation to the cytoplasm and proteasomal degradation.
  • 细胞定位

    Mitochondrion. The cleaved APEX2 is only detected in mitochondria (By similarity). Translocation from the cytoplasm to the mitochondria is mediated by ROS signaling and cleavage mediated by granzyme A. Tom20-dependent translocated mitochondrial APEX1 level is significantly increased after genotoxic stress and Nucleus. Nucleus, nucleolus. Nucleus speckle. Endoplasmic reticulum. Cytoplasm. Detected in the cytoplasm of B-cells stimulated to switch (By similarity). Colocalized with SIRT1 in the nucleus. Colocalized with YBX1 in nuclear speckles after genotoxic stress. Together with OGG1 is recruited to nuclear speckles in UVA-irradiated cells. Colocalized with nucleolin and NPM1 in the nucleolus. Its nucleolar localization is cell cycle dependent and requires active rRNA transcription. Colocalized with calreticulin in the endoplasmic reticulum. Translocation from the nucleus to the cytoplasm is stimulated in presence of nitric oxide (NO) and function in a CRM1-dependent manner, possibly as a consequence of demasking a nuclear export signal (amino acid position 64-80). S-nitrosylation at Cys-93 and Cys-310 regulates its nuclear-cytosolic shuttling. Ubiquitinated form is localized predominantly in the cytoplasm.
  • Target information above from: UniProt accession P27695 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 328 Human
    • Entrez Gene: 11792 Mouse
    • Omim: 107748 Human
    • SwissProt: P27695 Human
    • SwissProt: P28352 Mouse
    • Unigene: 73722 Human
    • Unigene: 203 Mouse
    • Unigene: 239117 Mouse
    • 别名

      • AP endonuclease 1 antibody
      • AP endonuclease class I antibody
      • AP lyase antibody
      • APE 1 antibody
      • APE antibody
      • APE-1 antibody
      • APEN antibody
      • APEX 1 antibody
      • APEX antibody
      • APEX nuclease (multifunctional DNA repair enzyme) 1 antibody
      • Apex nuclease 1 antibody
      • APEX nuclease antibody
      • APEX1 antibody
      • APEX1_HUMAN antibody
      • Apurinic endonuclease antibody
      • Apurinic-apyrimidinic endonuclease 1 antibody
      • Apurinic/apyrimidinic (abasic) endonuclease antibody
      • Apurinic/apyrimidinic endonuclease 1 antibody
      • Apurinic/apyrimidinic exonuclease antibody
      • APX antibody
      • BAP1 antibody
      • Deoxyribonuclease (apurinic or apyrimidinic) antibody
      • DNA (apurinic or apyrimidinic site) lyase antibody
      • DNA-(apurinic or apyrimidinic site) lyase, mitochondrial antibody
      • EC 4.2.99.18 antibody
      • HAP 1 antibody
      • HAP1 antibody
      • Human Apurinic endonuclease 1 antibody
      • MGC139790 antibody
      • Multifunctional DNA repair enzyme antibody
      • Redox factor 1 antibody
      • Redox factor-1 antibody
      • REF 1 antibody
      • REF 1 protein antibody
      • REF-1 antibody
      • REF1 antibody
      • REF1 protein antibody
      see all

    图片

    • Western blot - Anti-APE1 antibody [13B8E5C2] - ChIP Grade (ab194)
      Western blot - Anti-APE1 antibody [13B8E5C2] - ChIP Grade (ab194)
      All lanes : Anti-APE1 antibody [13B8E5C2] (ab194) at 1/2000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : APEX1 knockout HAP1 whole cell lysate
      Lane 3 : HepG2 whole cell lysate
      Lane 4 : HEK293 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 35.6 kDa
      Observed band size: 37 kDa why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab194 observed at 37 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.

      ab194 was shown to react with APEX1 in HAP1 wild-type cells in Western blot. Loss of signal was observed when APEX1 knockout sample was used. HAP1 wild-type and APEX1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab194 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody [13B8E5C2] - ChIP Grade (ab194)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody [13B8E5C2] - ChIP Grade (ab194)
      Ab194 staining Human normal placenta. Staining is localized to the nucleus.
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Western blot - Anti-APE1 antibody [13B8E5C2] - ChIP Grade (ab194)
      Western blot - Anti-APE1 antibody [13B8E5C2] - ChIP Grade (ab194)This image is courtesy of an anonymous Abreview
      Anti-APE1 antibody [13B8E5C2] (ab194) at 1/2000 dilution + Human fibroblast at 30 µg with Milk at 5 %

      Secondary
      ECL Mouse IgG,HRP - linked whole antibody (sheep) at 1/5000 dilution

      Developed using the ECL technique.

      Predicted band size: 35.6 kDa


      Exposure time: 10 seconds

      See Abreview

    实验方案

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (42)

    发表研究结果有使用 ab194?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab194 被引用在 42 文献中.

    • Tang Z  et al. Apurinic/apyrimidinic endonuclease 1/reduction-oxidation effector factor-1 (APE1) regulates the expression of NLR family pyrin domain containing 3 (NLRP3) inflammasome through modulating transcription factor NF-?B and promoting the secretion of inflammatory mediators in macrophages. Ann Transl Med 9:145 (2021). PubMed: 33569447
    • Zhang Y  et al. Arginine methylation of APE1 promotes its mitochondrial translocation to protect cells from oxidative damage. Free Radic Biol Med 158:60-73 (2020). PubMed: 32679368
    • Duan S  et al. Interaction between RECQL4 and OGG1 promotes repair of oxidative base lesion 8-oxoG and is regulated by SIRT1 deacetylase. Nucleic Acids Res 48:6530-6546 (2020). PubMed: 32432680
    • Wang LA  et al. Correlation of APE1 with VEGFA and CD163+ macrophage infiltration in bladder cancer and their prognostic significance. Oncol Lett 20:2881-2887 (2020). PubMed: 32782604
    • House NCM  et al. Site-specific targeting of a light activated dCas9-KillerRed fusion protein generates transient, localized regions of oxidative DNA damage. PLoS One 15:e0237759 (2020). PubMed: 33332350
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 14 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-APE1 antibody [13B8E5C2] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (fibroblasts)
    Permeabilization
    Yes - 0.3% Triton X-100 in blocking buffer
    Specification
    fibroblasts
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 20 2017

    Western blot abreview for Anti-APE1 antibody [13B8E5C2] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (fibroblast)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    30 µg
    Specification
    fibroblast
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 29 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-APE1 [13B8E5C2] antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Sample
    Human Cell (Human primary fibroblasts)
    Specification
    Human primary fibroblasts
    Permeabilization
    Yes - 0.2% Triton-X100
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 13 2013

    ELISA abreview for Anti-APE1 antibody [13B8E5C2] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Purified protein (purified APE1 protein)
    Specification
    purified APE1 protein
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Type
    Sandwich (Detection)
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Yu ran Lee

    Verified customer

    提交于 Dec 13 2012

    Immunoprecipitation abreview for Anti-APE1 antibody [13B8E5C2] - ChIP Grade

    Good
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (APE1 protein)
    Total protein in input
    0.1 µg
    Specification
    APE1 protein
    Immuno-precipitation step
    Protein A/G
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Yu ran Lee

    Verified customer

    提交于 Dec 10 2012

    Western blot abreview for Anti-APE1 antibody [13B8E5C2] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Liver, heart, kidney)
    Loading amount
    40 µg
    Specification
    Liver, heart, kidney
    Gel Running Conditions
    Reduced Denaturing (12% SDS PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Yu ran Lee

    Verified customer

    提交于 Dec 10 2012

    Immunoprecipitation abreview for Anti-APE1 antibody [13B8E5C2] - ChIP Grade

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Total protein in input
    200 µg
    Specification
    HeLa cells
    Immuno-precipitation step
    Protein A/G
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 07 2011

    Question

    I received this product and it is pink in colour.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 07 2013

    Answer

    Vials of this product may appear pink in colour as it is produced in ascites and not purified. Therefore this may give some discolouration as the product contains serum proteins.

    Read More

    Abcam Scientific Support

    回复于 Jun 07 2013

    Question

    I am inquiring about antibody ab194. I am interested if it is known the specific epitope for this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 24 2012

    Answer

    The exact epitope recognized by this antibody is not known, as it was raised to the full-length human protein and the epitope has not been mapped.

    Read More

    Abcam Scientific Support

    回复于 Dec 24 2012

    Question

    I am hoping to evaluate a number of antibodies to identify interactions with my protein of interest (Dbf4). I was just wondering if you supply sample sizes of the following to purchase:

    APEX1: Anti-APE1 antibody [13B8E5C2] - ChIP Grade (ab194)

    TOP1: Anti-Topoisomerase I antibody - ChIP Grade (ab3825)

    MPG: Anti-MPG antibody (ab55461)

    RCC2: Anti-RCC2 antibody (ab70787)

    TOP2: Anti-Topoisomerase II alpha antibody (ab12318)

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 09 2012

    Answer

    Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    If the product is to be used in an untested species or application, you may be eligible for our AbTrial program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Or you can find more information about this program at the following link:

    www.abcam.com/abtrial

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

    Abcam Scientific Support

    回复于 Nov 09 2012

    1-10 of 14 Abreviews or Q&A

    •  向后
    • 1
    • 2
    • 向前 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2023 艾博抗(上海)贸易有限公司版权所有