Anti-Apc5抗体(ab72516)
Key features and details
- Rabbit polyclonal to Apc5
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Apc5抗体
参阅全部 Apc5 一抗 -
描述
兔多克隆抗体to Apc5 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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免疫原
Synthetic peptide from a region between residue 705 and the C terminus (residue 755) of human Apc5 (NP_057321.2)
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阳性对照
- Whole cell lysate from Hela, 293T and NIH3T3 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72516于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/2500. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/500 - 1/2500. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the APC5 family.
Contains 4 TPR repeats. - Information by UniProt
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数据库链接
- Entrez Gene: 51433 Human
- Entrez Gene: 59008 Mouse
- Entrez Gene: 100171787 Orangutan
- Omim: 606948 Human
- SwissProt: Q9UJX4 Human
- SwissProt: Q8BTZ4 Mouse
- SwissProt: Q5RE52 Orangutan
- Unigene: 7101 Human
see all -
别名
- 2510006G12Rik antibody
- AA408751 antibody
- AA536819 antibody
see all
图片
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Western blot - Anti-Apc5 antibody (ab72516)All lanes : Anti-Apc5 antibody (ab72516) at 0.4 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-Apc5 antibody (ab72516)Immunoprecipitation/ Western Blot of Apc5.
Lane 1: ab72516 at 3µg/mg Hela whole cell lysate (1mg for IP, 20% of IP loaded). Lane 2: Control IgG.
ab72516 at 1µg/ml for WB. Chemiluminescence with an exposure time of 1 minute.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab72516 被引用在 1 文献中.
- Dai L et al. Dose-dependent proteomic analysis of glioblastoma cancer stem cells upon treatment with ?-secretase inhibitor. Proteomics : (2011). WB ; Human . PubMed: 21932445