Anti-Apc10抗体(ab106497)
Key features and details
- Rabbit polyclonal to Apc10
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-Apc10抗体
参阅全部 Apc10 一抗 -
描述
兔多克隆抗体to Apc10 -
宿主
Rabbit -
特异性
ab106497 is predicted not to cross-react with other APC family members. Two isoforms of Apc10 are known to exist; ab106497 will recognize both isoforms. -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse -
免疫原
A 16 amino acid synthetic peptide near the center of Human Apc10 (EAX05052).
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阳性对照
- Mouse heart tissue and lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106497于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa.
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| IHC-P |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 20 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa. |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the APC10 family.
Contains 1 DOC domain. - Information by UniProt
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数据库链接
- Entrez Gene: 68999 Mouse
- SwissProt: Q8K2H6 Mouse
- Unigene: 257445 Mouse
- Unigene: 420790 Mouse
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形式
It localises to Cytosol and nucleoplasm. -
别名
- ANAPC 10 antibody
- anapc10 antibody
- Anaphase promoting complex 10 antibody
see all
图片
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Western blot - Anti-Apc10 antibody (ab106497)Lane 1 : Anti-Apc10 antibody (ab106497) at 1 µg/ml
Lane 2 : Anti-Apc10 antibody (ab106497) at 2 µg/ml
All lanes : Mouse heart tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 21 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Apc10 antibody (ab106497)Immunofluorescence of Apc10 in mouse heart tissue with ab106497 at 20 ug/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc10 antibody (ab106497)ab106497, at 5 µg/ml, staining Apc10 in Mouse heart tissue by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab106497 尚未被引用在任何文献中。