Anti-Apc1抗体(ab133397)
Key features and details
- Rabbit polyclonal to Apc1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Apc1抗体
参阅全部 Apc1 一抗 -
描述
兔多克隆抗体to Apc1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic 17 amino acid peptide from near the C terminal of Human Apc1.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133397于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 216 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml.
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| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 216 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. |
靶标
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功能
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the APC1 family.
Contains 4 PC repeats. -
翻译后修饰
Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 64682 Human
- Entrez Gene: 17222 Mouse
- Omim: 608473 Human
- SwissProt: Q9H1A4 Human
- SwissProt: P53995 Mouse
- Unigene: 436527 Human
- Unigene: 277408 Mouse
- Unigene: 449185 Mouse
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别名
- ANAPC 1 antibody
- Anapc1 antibody
- Anaphase promoting complex subunit 1 antibody
see all
图片
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Western blot - Anti-Apc1 antibody (ab133397)Lane 1 : Anti-Apc1 antibody (ab133397) at 1 µg/ml
Lane 2 : Anti-Apc1 antibody (ab133397) at 2 µg/ml
Lane 1 : SK-N-SH cell lysate
Lane 2 : SK-N-SH cell lysate
Predicted band size: 216 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc1 antibody (ab133397)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labelling Apc1 with ab133397 at 5 µg/ml, using a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab133397 被引用在 3 文献中.
- Barbosa JAF et al. Human Immunodeficiency Virus Type 1 Vpr Mediates Degradation of APC1, a Scaffolding Component of the Anaphase-Promoting Complex/Cyclosome. J Virol 95:e0097120 (2021). PubMed: 34011540
- Lai XL et al. Apc gene suppresses intracranial aneurysm formation and rupture through inhibiting the NF-?B signaling pathway mediated inflammatory response. Biosci Rep 39:N/A (2019). PubMed: 30808715
- Sako K et al. Emi2 mediates meiotic MII arrest by competitively inhibiting the binding of Ube2S to the APC/C. Nat Commun 5:3667 (2014). PubMed: 24770399