AB317934

重组APC Anti-VDAC1/Porin + VDAC2抗体[EPR10852(B)] – Mitochondrial Loading Control

APC Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal VDAC1/Porin antibody - conjugated to APC.

查看别名

VDAC, VDAC1, Non-selective voltage-gated ion channel VDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, Voltage-dependent anion-selective channel protein 1, VDAC-1, hVDAC1

不同偶联物与剂型 (7)

HRP

HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control
578 PE

PE Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR10852(B)

亚型

IgG

偶联物

APC

激发波长/发射波长

Ex: 650nm, Em: 660nm

不含载体蛋白

应用

Target Binding Affinity, Antibody Labelling

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The VDAC1/Porin and VDAC2 proteins also known as voltage-dependent anion-selective channel 1 and 2 serve as important pore-forming proteins within the mitochondrial outer membrane. Each VDAC protein has a molecular mass of about 30-35 kDa. Commonly referred to as the 'porin hub' due to their central role in metabolite exchange VDAC1 and VDAC2 facilitate the transport of ions and small molecules between the mitochondria and cytoplasm regulating energy production and cellular metabolism. They express widely across various tissues highlighting their essential role in cellular functions.

Biological function summary

Voltage-dependent anion channels like VDAC1/Porin and VDAC2 modulate the exchange of ions and metabolic substrates playing a significant role in apoptosis and the maintenance of mitochondrial functions. As integral parts of mitochondrial complexes they interact with proteins such as hexokinase which anchors to VDAC1 and influences glycolytic flux linking energy production with apoptotic signaling pathways. This positions VDAC proteins as key regulators of cellular energy homeostasis.

Pathways

VDAC1/Porin and VDAC2 engage prominently in the apoptotic and mitochondrial permeability transition pathways. In the apoptosis pathway they interact with anti-apoptotic proteins like Bcl-xl and pro-apoptotic members like Bax highlighting their dual role in cell survival and death. These interactions highlight the proteins’ contribution to mitochondrial outer membrane permeabilization (MOMP) a critical event in the release of cytochrome c and subsequent apoptosome formation further integrating them into broader signaling networks.

Dysfunctions in VDAC1/Porin and VDAC2 link to neurodegenerative diseases and cancer. Aberrant VDAC1 activity connects with Alzheimer's disease due to its role in mitochondrial dysfunction affecting amyloid-beta peptide accumulation and neuronal cell death. In cancer VDAC overexpression associates with altered cellular metabolism and resistance to apoptosis positioning these proteins as potential therapeutic targets. Interactions with proteins such as hexokinase and Bcl-2 family members further emphasize their role in these pathological processes.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane (PubMed : 10661876, PubMed : 11845315, PubMed : 18755977, PubMed : 30061676, PubMed : 8420959). The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis (PubMed : 10661876, PubMed : 11845315, PubMed : 18755977, PubMed : 8420959). It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV (PubMed : 10661876, PubMed : 18755977, PubMed : 8420959). The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed : 18755977, PubMed : 8420959). Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed : 18755977, PubMed : 31015432). In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed : 32047033). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed : 15033708, PubMed : 25296756). May mediate ATP export from cells (PubMed : 30061676). Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix (By similarity). Mediates cytochrome c efflux (PubMed : 20230784).. Catalyzes the scrambling of phospholipids across the outer mitochondrial membrane; the mechanism is unrelated to channel activity and is capable of translocating both anionic and zwitterionic phospholipids.

See full target information VDAC1

其他靶点

Voltage-dependent anion-selective channel protein 2

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重组APC Anti-VDAC1/Porin + VDAC2抗体[EPR10852(B)] – Mitochondrial Loading Control