重组APC Anti-SIGLEC8抗体[EPR24419-56] (ab318168)
![Flow Cytometry (Intracellular) - APC Anti-SIGLEC8 antibody [EPR24419-56] (ab318168)](https://www.abcam.cn/ps/products/318/ab318168/Images/ab318168-1-apc-anti-siglec8-antibody-epr24419-56-flow-cytometry-isotype.jpg "Flow Cytometry (Intracellular) - APC Anti-SIGLEC8 antibody [EPR24419-56] (ab318168)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EPR24419-56] to SIGLEC8
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
Related conjugates and formulations
概述
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产品名称
APC Anti-SIGLEC8抗体[EPR24419-56]
参阅全部 SIGLEC8 一抗 -
描述
APC兔单克隆抗体[EPR24419-56] to SIGLEC8 -
宿主
Rabbit -
偶联物
APC. Ex: 645nm, Em: 660nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (Intra): HEK-293T cells transfected with a human SIGLEC8 expression vector containing a myc-his tag.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR24419-56 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab318168于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/5000.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/5000. |
靶标
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功能
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. -
组织特异性
Expressed specifically on eosinophils. -
序列相似性
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
结构域
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 27181 Human
- Omim: 605639 Human
- SwissProt: Q9NYZ4 Human
- Unigene: 447899 Human
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别名
- CD329 antigen antibody
- CDw329 antibody
- SAF 2 antibody
see all
图片
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Flow Cytometry (Intracellular) - APC Anti-SIGLEC8 antibody [EPR24419-56] (ab318168)
Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / HEK-293T (human embryonic kidney) cells transfected with a human SIGLEC8 expression vector containing a myc-his tag (Middle) / 293T transfected with an empty vector containing a myc-His tag (Right) cells labelling SIGLEC8 with ab318168 at 1/5000 dilution (0.01ug) / Middle and Right (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Live cells were surface stained with ab318168, then fixed with 2% PFA followed by intracellular staining with Myc-tag conjugated to Alexa Fluor® 488.
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![APC Anti-SIGLEC8 antibody [EPR24419-56] (ab318168)](https://www.abcam.cn/ps/products/318/ab318168/Images/ab318168-2-benefitsofrecombinantantibodies.jpg)
APC Anti-SIGLEC8 antibody [EPR24419-56] (ab318168)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab318168 尚未被引用在任何文献中。