JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB319206

重组APC Anti-PMCA1抗体[EPR12029]

APC Anti-PMCA1 antibody [EPR12029]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal PMCA1 antibody - conjugated to APC.

查看别名

PMCA1, ATP2B1, Plasma membrane calcium-transporting ATPase 1, Plasma membrane calcium ATPase isoform 1, Plasma membrane calcium pump isoform 1

不同偶联物与剂型 (5)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12029

亚型

IgG

偶联物

APC

激发波长/发射波长

Ex: 650nm, Em: 660nm

不含载体蛋白

No

应用

Antibody Labelling, Target Binding Affinity

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The plasma membrane calcium ATPase 1 (PMCA1) also known by its gene name ATP2B1 is an essential protein responsible for the regulation of intracellular calcium levels. Mechanically PMCA1 functions as a calcium pump actively transporting calcium ions out of the cell using ATP hydrolysis. This protein has a molecular mass of approximately 135 kDa. PMCA1 is widely expressed in many tissues but shows higher expression in excitable cells such as neurons and muscle cells where rapid calcium signaling is critical.
Biological function summary

PMCA1 plays a fundamental role in maintaining calcium homeostasis which is important for many cellular processes including muscle contraction nerve impulse transmission and cell signaling. It functions independently but in some contexts it may interact with other proteins to modulate its activity. The protein helps prevent toxic accumulation of calcium inside cells therefore protecting cellular integrity and function.

Pathways

PMCA1 is integral to the calcium signaling pathway where it helps regulate cellular responses to fluctuating calcium levels. It interacts with other key proteins such as calmodulin which can enhance its activity in response to increased intracellular calcium. PMCA1 also links to the phosphatidylinositol signaling pathway where its function assists in the propagation of signaling cascades that manage processes like growth and metabolism.

PMCA1 has associations with conditions impacting calcium regulation such as hypertension and cardiovascular diseases. Dysfunctional regulation of PMCA1 can lead to abnormal calcium homeostasis contributing to such disorders. Furthermore PMCA1's interaction with proteins like the sodium-calcium exchanger (NCX) suggests a complex regulatory network that when disrupted may trigger or exacerbate disease states.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Catalyzes the hydrolysis of ATP coupled with the transport of calcium from the cytoplasm to the extracellular space thereby maintaining intracellular calcium homeostasis (PubMed : 35358416). Plays a role in blood pressure regulation through regulation of intracellular calcium concentration and nitric oxide production leading to regulation of vascular smooth muscle cells vasoconstriction. Positively regulates bone mineralization through absorption of calcium from the intestine. Plays dual roles in osteoclast differentiation and survival by regulating RANKL-induced calcium oscillations in preosteoclasts and mediating calcium extrusion in mature osteoclasts (By similarity). Regulates insulin sensitivity through calcium/calmodulin signaling pathway by regulating AKT1 activation and NOS3 activation in endothelial cells (PubMed : 29104511). May play a role in synaptic transmission by modulating calcium and proton dynamics at the synaptic vesicles.
See full target information ATP2B1
style
left-column
style
left-column
style
right-column

搭配使用产品

Assay Kits

AB102505

Calcium Assay Kit (Colorimetric)

Functional Studies - Calcium Assay Kit (Colorimetric) (AB102505)

  • 5
  • 4
Cell Lines & Lysates

AB265561

Human ATP2B1 (PMCA1) knockout HeLa cell line

Advanced Validation

Western blot - Human ATP2B1 (PMCA1) knockout HeLa cell line (AB265561)

  • 0
  • 0
Cell Lines & Lysates

AB257365

Human ATP2B1 (PMCA1) knockout HeLa cell lysate

Western blot - Human ATP2B1 (PMCA1) knockout HeLa cell lysate (AB257365)

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com