重组APC Anti-PI 3 Kinase p85 alpha抗体[EPR18702] (ab225369)
![Flow Cytometry (Intracellular) - APC Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (ab225369)](https://www.abcam.cn/ps/products/225/ab225369/Images/ab225369-307469-ab225369FLOW1.jpg "Flow Cytometry (Intracellular) - APC Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (ab225369)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EPR18702] to PI 3 Kinase p85 alpha
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
Related conjugates and formulations
概述
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产品名称
APC Anti-PI 3 Kinase p85 alpha抗体[EPR18702]
参阅全部 PI 3 Kinase p85 alpha 一抗 -
描述
APC兔单克隆抗体[EPR18702] to PI 3 Kinase p85 alpha -
宿主
Rabbit -
偶联物
APC. Ex: 645nm, Em: 660nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (intra): HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR18702 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225369于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/5000.
The cellular localisation of this product has been verified in ICC/IF. |
| 说明 |
|---|
|
Flow Cyt (Intra)
1/5000. The cellular localisation of this product has been verified in ICC/IF. |
靶标
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功能
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. -
组织特异性
Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level). -
序列相似性
Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
结构域
The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef. -
翻译后修饰
Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
Phosphorylated. Dephosphorylated by PTPRJ. - Information by UniProt
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数据库链接
- Entrez Gene: 5295 Human
- Entrez Gene: 18708 Mouse
- Entrez Gene: 25513 Rat
- Omim: 171833 Human
- SwissProt: P27986 Human
- SwissProt: P26450 Mouse
- SwissProt: Q63787 Rat
- Unigene: 132225 Human
see all -
别名
- GRB1 antibody
- p85 alpha antibody
- p85 antibody
see all
图片
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Flow Cytometry (Intracellular) - APC Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (ab225369)
Overlay histogram showing Jurkat cells stained with ab225369 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225369, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
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![APC Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (ab225369)](https://www.abcam.cn/ps/products/225/ab225369/Images/ab225369-3-benefits-of-recombinant-antibodies.png)
APC Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (ab225369)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab225369 尚未被引用在任何文献中。
