APC Anti-PDGFR beta抗体[18A2] (ab119861)
![Flow Cytometry - APC Anti-PDGFR beta antibody [18A2] (ab119861)](https://www.abcam.cn/ps/products/119/ab119861/Images/ab119861-550878-apc-anti-pdgfr-beta-antibody-18a2flow-cyto-pfgf-rb-transfected-3t3-cells.jpg "Flow Cytometry - APC Anti-PDGFR beta antibody [18A2] (ab119861)")
Key features and details
- APC Mouse monoclonal [18A2] to PDGFR beta
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
概述
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产品名称
APC Anti-PDGFR beta抗体[18A2]
参阅全部 PDGFR beta 一抗 -
描述
APC小鼠单克隆抗体[18A2] to PDGFR beta -
宿主
Mouse -
偶联物
APC. Ex: 645nm, Em: 660nm -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human PDGFR beta. CD140b-transfected NIH 3T3 cells
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阳性对照
- Human blood cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading... -
纯度
Size exclusion -
克隆
单克隆 -
克隆编号
18A2 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119861于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 10µl for 106 cells.
The reagent is designed for Flow Cytometry analysis of human blood cells using 10µl reagent / 100µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
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Flow Cyt
Use 10µl for 106 cells. The reagent is designed for Flow Cytometry analysis of human blood cells using 10µl reagent / 100µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2. -
疾病相关
Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5159 Human
- Omim: 173410 Human
- SwissProt: P09619 Human
- Unigene: 509067 Human
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别名
- Beta platelet derived growth factor receptor antibody
- Beta-type platelet-derived growth factor receptor antibody
- CD 140B antibody
see all
图片
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Flow Cytometry - APC Anti-PDGFR beta antibody [18A2] (ab119861)
Flow cytometry analysis of PFGF-RB transfected 3T3 cells (upper panel)/on-transefected 3T3 cells (lower panel) staining PDGFR beta with ab119861 at 5 µg/ml (Red). Level of non-specific binding was assessed using Mouse IgG1 isotype control APC antibody under same conditions (concentration in sample 5 μg/ml black-dashed histogram).
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![Flow Cytometry - APC Anti-PDGFR beta antibody [18A2] (ab119861)](https://www.abcam.cn/ps/products/119/ab119861/Images/ab119861-310914-ab119861FC.jpg)
Flow Cytometry - APC Anti-PDGFR beta antibody [18A2] (ab119861)Flow Cytometry surface staining of human CD140b / PDGF-R Beta stable transfectants, labeling CD5 with ab232704 (10 μl reagent / 100 μl of whole blood). Gating strategy - singlet gate.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab119861 被引用在 1 文献中.
- Fekete N et al. GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC. PLoS One 7:e43255 (2012). PubMed: 22905242