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APC Anti-NCAM1抗体[MEM-188] (ab28335)

Reviews (1)Q&A (3)
Flow Cytometry - APC Anti-NCAM1 antibody [MEM-188] (ab28335)
  • Flow Cytometry - APC Anti-NCAM1 antibody [MEM-188] (ab28335)
  • Flow Cytometry - APC Anti-NCAM1 antibody [MEM-188] (ab28335)

Key features and details

  • APC Mouse monoclonal [MEM-188] to NCAM1
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: APC. Ex: 645nm, Em: 660nm
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

Biotin FITC PE PE/Cy7® PerCP Unconjugated

概述

  • 产品名称

    APC Anti-NCAM1抗体[MEM-188]
    参阅全部 NCAM1 一抗

  • 描述

    APC小鼠单克隆抗体[MEM-188] to NCAM1

  • 宿主

    Mouse

  • 偶联物

    APC. Ex: 645nm, Em: 660nm

  • 特异性

    ab28335 recognises an isoform of NCAM characteristic for leucocytes. Reactivity with other NCAM isoforms has not been tested.

  • 经测试应用

    适用于: Flow Cytmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Tissue, cells or virus corresponding to Human NCAM1. KG-1 human acute myelogenous leukaemia cell line

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab28335于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (1)
Use at an assay dependent concentration.

Use 10 ul per 100 ul of whole blood. ab91364 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
说明
Flow Cyt
Use at an assay dependent concentration.

Use 10 ul per 100 ul of whole blood. ab91364 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

  • 序列相似性

    Contains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

  • 细胞定位

    Secreted and Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
      • antigen recognized by monoclonal antibody 5.1H11 antibody
      • CD56 antibody
      • cell adhesion molecule, neural, 1 antibody
      • MSK 39 antibody
      • MSK39 antibody
      • N-CAM-1 antibody
      • NCAM 1 antibody
      • NCAM antibody
      • NCAM C antibody
      • NCAM-1 antibody
      • NCAM1 antibody
      • NCAM1_HUMAN antibody
      • NCAMC antibody
      • Neural cell adhesion molecule 1 antibody
      • Neural cell adhesion molecule NCAM antibody
      • OTTHUMP00000235666 antibody
      see all

    图片

    • Flow Cytometry - APC Anti-NCAM1 antibody [MEM-188] (ab28335)
      Flow Cytometry - APC Anti-NCAM1 antibody [MEM-188] (ab28335)

      Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD3 (UCHT1) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and ab28335 (10 μl reagent / 100 μl of peripheral whole blood).

    • Flow Cytometry - APC Anti-NCAM1 antibody [MEM-188] (ab28335)
      Flow Cytometry - APC Anti-NCAM1 antibody [MEM-188] (ab28335)

      Flow cytometry surface staining pattern of human peripheral whole blood stained using ab28335 (10 μl reagent / 100 μl of peripheral whole blood).

    • Flow Cytometry - APC Anti-NCAM1 antibody [MEM-188] (ab28335)
      Flow Cytometry - APC Anti-NCAM1 antibody [MEM-188] (ab28335)

      Separation of human CD56 positive CD3 negative NK cells (red-filled) from CD56 negative CD3 positive T cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab28335 (10 μl reagent / 100 μl of peripheral whole blood).

    文献 (0)

    发表研究结果有使用 ab28335？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab28335 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-4 of 4 Abreviews or Q&A

    Flow Cytometry abreview for Anti-NCAM antibody [MEM-188] (Allophycocyanin)

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (PBMC)
    Specification
    PBMC
    Preparation
    Cell harvesting/tissue preparation method: Ficoll-Hypaque gradient
    Sample buffer: PBS
    Permeabilization
    No
    Gating Strategy
    lymphocytes
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 24 2013

    Question

    ab15160:
    IHC-Fr with mouse skin
    no staining
    Ab: 1/50 - 1/500
    fix: 10 min PFA or 2h PFA (no difference seen)
    2nd: works well
    block: BSA + gelatin+ NGS+NDS (uses regulary and works well)

    ab28335:
    flow cytometry
    uses GFP reporter mouse line (GFP: x-axis)
    cells should highly express NCAM, but sees no shift for APC (y-axis)
    single cell suspension o tissue
    Ab: 30 min, no fixation, read at cytometer
    Ab titration between 2ul and 60ul / 10^6 cells

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry these 2 products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx (ab15160, new lot and ab14047).

    To check the status of the order please contact our Customer Service team and reference this number.
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
    I wish you the best of luck with your research.

    Read More

    Question

    Hi, I been using the Anti-NCAM [MEM-188] (APC) ab28335, but now I want to try the biotinilated Anti-NCAM [MEM-188] Biotin (ab25640)
    My question is, in the webpage, the ab28335 reacts with human and mice, but the ab25640 only with human? I need the Ab for mice, so I need to be sure that the biotinilated ab (ab25640) will work on mice, I believe is the same clone... could you clarify this?
    Could you also give the current availability of the ab25640?
    Thanks,

    Read More
    Answer

    Thank you very much for contacting us with your question.

    We do expectall antibodies from the same clone tohave the same cross-reactivity, so Ab25640 should react with mouse samples despite the difference in conjugation between it and Ab28335. We don't add a species to the datasheet for a particular product untilit is tested, and Ab25640 hasn't yet been tested with mouse samples so only human is listed.

    Since Ab25640 hasn't specifically been tested with mouse samples, you are eligible for our testing discount program if you'd like to try it. For this program, you would first purchase ab25640, test it with mouse samples, and submit your feedback to us in an online Abreview. In exchange we will give you one free primary antibody on a future purchase. Please let me know if you're interested in this program and I can set this up for you.

    We do not currently hold stock of Ab25640, and we expect it to arrive within 1-2 weeks of placing an order.

    I hope that this information is useful, but please let me know if you have further questions or need anything else and I'll be happy to help.

    Read More

    Question

    Ab28335 and ab25358 are the same clone but ab28335 gave no signal in flow cytometry and ab25358 gave a strong signal. How do we know which antibody is producing the correct data?

    Read More
    Answer

    Thank you for your call today and for letting us know about this issue.

    As we discussed, please keep me updated with the results after using positive and negative control cell lines. I will also try to find any additional information that might explain the discrepancy between these antibodies.

    Please let me know if you have any further questions or if there is anything else that we can do for you.

    Read More

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