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AB275181

重组APC Anti-Junctional Adhesion Molecule 1/JAM-A抗体[EPR23246-275]

APC Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275]

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Rabbit Recombinant Monoclonal Junctional Adhesion Molecule 1/JAM-A antibody - conjugated to APC. Suitable for Flow Cyt, ICC/IF and reacts with Mouse samples.

查看别名

CD321, Jam1, Jcam, Jcam1, F11r, Junctional adhesion molecule A, JAM-A, Junctional adhesion molecule 1, JAM-1

1 Images
Flow Cytometry - APC Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] (AB275181)
  • Flow Cyt

Lab

Flow Cytometry - APC Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] (AB275181)

Flow cytometry overlay histogram showing (left) Bend.3 positive cells and right negative Neuro2a cells stained with ab275181 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab275181) (1x 106 in 100μl at 5μg/ml (1/100)) for 30 min on ice.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (ab232814) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

不同偶联物与剂型 (5)

  • Unconjugated

    Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275]

  • Carrier free

    Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275]

  • 578 PE

    PE Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR23246-275

亚型

IgG

偶联物

APC

激发波长/发射波长

Ex: 650nm, Em: 660nm

不含载体蛋白

No

反应种属

Mouse, Mouse

应用

Flow Cyt, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Assay Kits

AB277079

Human JAM-A ELISA Kit (Junctional Adhesion Molecule)

Recombinant|SimpleStep

Sandwich ELISA - Human JAM-A ELISA Kit (Junctional Adhesion Molecule) (AB277079)

  • 0
  • 0
Assay Kits

AB178013

Human IL-6 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

  • 5
  • 2
Proteins & Peptides

AB126670

Recombinant Human Junctional Adhesion Molecule 1/JAM-A protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Junctional Adhesion Molecule 1/JAM-A protein (His tag N-Terminus) (AB126670)

  • 0
  • 0
Assay Kits

AB181421

Human TNF alpha ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

  • 5
  • 4
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Junctional Adhesion Molecule 1 also known as JAM-A or JAM-A protein is a member of the immunoglobulin superfamily. It has a mass of roughly 32 kDa. This molecule functions in tight junctions of epithelial and endothelial cells playing an important role in regulating paracellular permeability. JAM-A is expressed in tissues where tight junctions are present including the skin gastrointestinal tract and endothelia throughout the body.
Biological function summary

JAM-A functions as a cell adhesion molecule influencing processes like leukocyte transmigration and epithelial barrier formation. It does not act alone but rather forms complexes with other proteins at cell junctions. JAM-A interacts with other junctional proteins like occludin and claudins contributing to the structure and function of tight junctions.

Pathways

JAM-A participates in cellular processes involving inflammation and cell migration. It is an integral part of the signal transduction pathways that regulate leukocyte migration an important aspect of immune response. JAM-A is also associated with G-protein coupled receptors in these pathways highlighting its role in regulating intracellular signaling that affects cell adhesion and migration.

JAM-A has connections with cancer progression and cardiovascular diseases. Its overexpression has been linked to several cancers including breast cancer due to its role in enhancing cell migration and invasion. In cardiovascular diseases JAM-A interacts with platelet-endothelial cell adhesion molecule-1 (PECAM-1) influencing the inflammatory response within vascular tissues. Understanding these interactions can aid in developing therapeutic strategies targeting JAM-A in related diseases.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed : 11447115). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (PubMed : 11447115). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (PubMed : 9660867). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (By similarity). Involved in platelet activation (By similarity).
See full target information F11r
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Abcam Product Promise

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