重组APC Anti-IRS2抗体[EPR904(2)] (ab221281)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EPR904(2)] to IRS2
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
Related conjugates and formulations
概述
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产品名称
APC Anti-IRS2抗体[EPR904(2)]
参阅全部 IRS2 一抗 -
描述
APC兔单克隆抗体[EPR904(2)] to IRS2 -
宿主
Rabbit -
偶联物
APC. Ex: 645nm, Em: 660nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (intra): HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR904(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab221281于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/500.
The cellular localisation of this product has been verified in ICC/IF. |
说明 |
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Flow Cyt (Intra)
1/500. The cellular localisation of this product has been verified in ICC/IF. |
靶标
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功能
May mediate the control of various cellular processes by insulin. -
序列相似性
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm > cytosol. - Information by UniProt
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数据库链接
- Entrez Gene: 8660 Human
- Entrez Gene: 384783 Mouse
- Entrez Gene: 29376 Rat
- Omim: 600797 Human
- SwissProt: Q9Y4H2 Human
- SwissProt: P81122 Mouse
- Unigene: 442344 Human
- Unigene: 407207 Mouse
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别名
- Insulin receptor substrate 2 antibody
- IRS 2 antibody
- IRS-2 antibody
see all
图片
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ab221281 AP-0037167
Overlay histogram showing HeLa cells stained with ab221281 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab221281, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (ab232814) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
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