重组APC Anti-IL-21R抗体[EPR23637-53]

• Flow Cyt

Lab

Flow Cytometry - APC Anti-IL-21R antibody [EPR23637-53] (AB313961)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab313961 (right) or APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab313961 or APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 10⁶ in 100 μl at 1 μg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with CD19.

Acquisition of >30000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

Events were gated on viable lymphocytes.