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AB316298

重组APC Anti-Glucose Transporter GLUT1抗体[EPR3915]

APC Anti-Glucose Transporter GLUT1 antibody [EPR3915]

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Knockout Tested Rabbit Recombinant Monoclonal Glucose Transporter GLUT1 antibody - conjugated to APC. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

查看别名

GLUT1, SLC2A1, HepG2 glucose transporter, GLUT-1

1 Images
Flow Cytometry (Intracellular) - APC Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB316298)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB316298)

Flow cytometry overlay histogram showing wild-type A549 (green line) and GLUT1 knockout A549 stained with ab316298 (magenta line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab316298) (1x 10E6 in 100μl at 0.04 μg/ml (1/12500)) for 30min at 22°C.

Isotype control antibody APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814) was used at the same concentration and conditions as the primary antibody (wild-type A549 - black line, GLUT1 knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

不同偶联物与剂型 (11)

  • Unconjugated

    Anti-Glucose Transporter GLUT1 antibody [EPR3915]

  • Carrier free

    Anti-Glucose Transporter GLUT1 antibody [EPR3915] - Low endotoxin, Azide free

  • Carrier free

    Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free

  • 421 Alexa Fluor® 405

    Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915]

  • Biotin

    Biotin Anti-Glucose Transporter GLUT1 antibody [EPR3915]

  • 519 FITC

    FITC Anti-Glucose Transporter GLUT1 antibody [EPR3915]

  • HRP

    HRP Anti-Glucose Transporter GLUT1 antibody [EPR3915]

  • 578 PE

    PE Anti-Glucose Transporter GLUT1 antibody [EPR3915]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3915

亚型

IgG

偶联物

APC

激发波长/发射波长

Ex: 650nm, Em: 660nm

不含载体蛋白

No

反应种属

Human, Human, Mouse, Rat

应用

IHC-P, ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Assay Kits

AB136955

Glucose Uptake Assay Kit (Colorimetric)

Functional Studies - Glucose Uptake Assay Kit (Colorimetric) (AB136955)

  • 5
  • 1
Primary Antibodies

AB216385

Alexa Fluor® 594 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB216385)

  • 4
  • 1
Primary Antibodies

AB171449

Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9]

KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (AB171449)

  • 5
  • 4
Primary Antibodies

AB305048

Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66] (AB305048)

  • 5
  • 1
Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/12500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Glucose Transporter GLUT1 also known as SLC2A1 is an important protein responsible for the transport of glucose across cell membranes. The GLUT1 transporter has a molecular weight of approximately 55 kDa. This protein is highly expressed in erythrocytes endothelial cells lining blood vessels and in the blood-brain barrier. Its primary role is to facilitate the basal glucose uptake necessary for cellular metabolism particularly in tissues where glucose is a critical energy source.
Biological function summary

This glucose transporter plays a significant role in maintaining glucose homeostasis in the human body. GLUT1 functions independently and not as part of a complex. It ensures that glucose is available to cells with high metabolic demands including the brain and red blood cells where it remains important for survival and function. Its expression level can be influenced by various factors including hypoxia and insulin.

Pathways

GLUT1 is involved in the glycolysis and hypoxia-related pathways. It supports the glycolytic pathway by ensuring a sufficient supply of glucose to the cells which is then metabolized to produce ATP. Additionally during hypoxic conditions GLUT1 expression can increase aligning with proteins like HIF-1α which helps cells adapt by modifying their metabolism. This coordinated regulation permits cells to adjust their energy systems according to the oxygen availability.

GLUT1 is implicated in glucose transporter type 1 deficiency syndrome (GLUT1 DS) and various forms of cancer. GLUT1 DS results from inadequate glucose transport into the brain presenting neurological symptoms due to energy deficiency. In cancer overexpression of GLUT1 links to increased glucose uptake and tumor growth a condition known to involve proteins like hexokinase. These associations underline GLUT1's contribution to both genetic defects and metabolic shifts in cancerous tissues.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Facilitative glucose transporter, which is responsible for constitutive or basal glucose uptake (PubMed : 10227690, PubMed : 10954735, PubMed : 18245775, PubMed : 19449892, PubMed : 25982116, PubMed : 27078104, PubMed : 32860739). Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses (PubMed : 18245775, PubMed : 19449892). Most important energy carrier of the brain : present at the blood-brain barrier and assures the energy-independent, facilitative transport of glucose into the brain (PubMed : 10227690). In association with BSG and NXNL1, promotes retinal cone survival by increasing glucose uptake into photoreceptors (By similarity). Required for mesendoderm differentiation (By similarity).
See full target information SLC2A1
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