重组APC Anti-Flotillin 1抗体[EPR6041] (ab225164)
![Flow Cytometry (Intracellular) - APC Anti-Flotillin 1 antibody [EPR6041] (ab225164)](https://www.abcam.cn/ps/products/225/ab225164/Images/ab225164-312647-anti-flotillin-1-antibody-epr6041-allophycocyanin-flow-cytometry.jpg "Flow Cytometry (Intracellular) - APC Anti-Flotillin 1 antibody [EPR6041] (ab225164)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EPR6041] to Flotillin 1
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
Related conjugates and formulations
概述
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产品名称
APC Anti-Flotillin 1抗体[EPR6041]
参阅全部 Flotillin 1 一抗 -
描述
APC兔单克隆抗体[EPR6041] to Flotillin 1 -
宿主
Rabbit -
偶联物
APC. Ex: 645nm, Em: 660nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (intra): A431 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6041 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225164于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
The cellular localisation of this product has been verified in ICC/IF. |
| 说明 |
|---|
|
Flow Cyt (Intra)
1/500. The cellular localisation of this product has been verified in ICC/IF. |
靶标
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功能
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. -
序列相似性
Belongs to the band 7/mec-2 family. Flotillin subfamily. -
细胞定位
Cell membrane. Membrane > caveola. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 10211 Human
- Entrez Gene: 14251 Mouse
- Entrez Gene: 64665 Rat
- Omim: 606998 Human
- SwissProt: O75955 Human
- SwissProt: O08917 Mouse
- SwissProt: Q9Z1E1 Rat
- Unigene: 179986 Human
see all -
别名
- FLOT 1 antibody
- FLOT1 antibody
- FLOT1_HUMAN antibody
see all
图片
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Flow Cytometry (Intracellular) - APC Anti-Flotillin 1 antibody [EPR6041] (ab225164)
Overlay histogram showing A431 cells stained with ab225164 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225164, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (ab232814) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
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![APC Anti-Flotillin 1 antibody [EPR6041] (ab225164)](https://www.abcam.cn/ps/products/225/ab225164/Images/ab225164-3-benefits-of-recombinant-antibodies.png)
APC Anti-Flotillin 1 antibody [EPR6041] (ab225164)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab225164 尚未被引用在任何文献中。
