APC Anti-CD45抗体[MEM-28] (ab28106)
![Flow Cytometry - APC Anti-CD45 antibody [MEM-28] (ab28106)](https://www.abcam.cn/ps/products/28/ab28106/Images/ab28106-449631-anti-cd45-antibody-flow-cytometry-peripheral-blood-human.jpg "Flow Cytometry - APC Anti-CD45 antibody [MEM-28] (ab28106)")
Key features and details
- APC Mouse monoclonal [MEM-28] to CD45
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
APC Anti-CD45抗体[MEM-28]
参阅全部 CD45 一抗 -
描述
APC小鼠单克隆抗体[MEM-28] to CD45 -
宿主
Mouse -
偶联物
APC. Ex: 645nm, Em: 660nm -
特异性
ab28106 reacts with all alternative forms of human CD45 antigen -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD45. Human thymocytes and T lymphocytes
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阳性对照
- Flow Cyt: Human peripheral blood cells.
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常规说明
The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
纯度
IgG fraction -
克隆
单克隆 -
克隆编号
MEM-28 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
- FITC Anti-CD45 antibody [MEM-28], prediluted (ab134199)
- PE Anti-CD45 antibody [MEM-28], prediluted (ab134202)
- PerCP/Cy5.5® Anti-CD45 antibody [MEM-28] (ab157309)
- APC/Cy7® Anti-CD45 antibody [MEM-28] (ab197130)
- PE/DyLight™ 594 Anti-CD45 antibody [MEM-28] (ab223183)
- Alexa Fluor® 647 Anti-CD45 antibody [MEM-28] (ab239276)
- PE/Cy7® Anti-CD45 antibody [MEM-28] (ab239317)
- Biotin Anti-CD45 antibody [MEM-28] (ab28107)
- PerCP Anti-CD45 antibody [MEM-28] (ab65952)
- Anti-CD45 antibody [MEM-28] (ab8216)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28106于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | (1) |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Flow Cyt
Use at an assay dependent concentration. |
靶标
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功能
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. -
疾病相关
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
结构域
The first PTPase domain interacts with SKAP1. -
翻译后修饰
Heavily N- and O-glycosylated. -
细胞定位
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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数据库链接
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
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别名
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
图片
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Flow Cytometry - APC Anti-CD45 antibody [MEM-28] (ab28106)
Flow cytometry surface staining pattern of human peripheral whole blood stained using ab28106 at (10 μl reagent / 100 μl of peripheral whole blood).
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![Flow Cytometry - APC Anti-CD45 antibody [MEM-28] (ab28106)](https://www.abcam.cn/ps/products/28/ab28106/Images/ab28106-168799-anti-cd45-antibody-mem-28-allophycocyanin-flow-cytometry.jpg)
Flow Cytometry - APC Anti-CD45 antibody [MEM-28] (ab28106)This image is courtesy of an anonymous Abreviewab28106 staining the CD45 in Human PBMCs by Flow Cytometry. Cells were prepared by Ficoll-Hypaque isolation of PBMCs from buffy coat. The sample was incubated with the primary antibody (1/25 in PBS + 2% Human serum and 1mM EDTA) for 25 minutes at 4°C.
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![Flow Cytometry - APC Anti-CD45 antibody [MEM-28] (ab28106)](https://www.abcam.cn/ps/products/28/ab28106/Images/ab28106-449630-anti-cd45-antibody-flow-cytometry-lymphocytes-human.jpg)
Flow Cytometry - APC Anti-CD45 antibody [MEM-28] (ab28106)Separation of human CD45 positive lymphocytes (red-filled) from human CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab28106(10 μl reagent / 100 μl of peripheral whole blood).
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab28106 被引用在 6 文献中.
- Deliorman M et al. Affinity-Based Microfluidics Combined with Atomic Force Microscopy for Isolation and Nanomechanical Characterization of Circulating Tumor Cells. Methods Mol Biol 2679:41-66 (2023). PubMed: 37300608
- Shen H et al. Antibody-engineered red blood cell interface for high-performance capture and release of circulating tumor cells. Bioact Mater 11:32-40 (2022). PubMed: 34938910
- Zhang Q et al. Isolation of circulating tumor cells and detection of EGFR mutations in patients with non-small-cell lung cancer. Oncol Lett 17:3799-3807 (2019). PubMed: 30881500
- Sundqvist M et al. Similarities and differences between the responses induced in human phagocytes through activation of the medium chain fatty acid receptor GPR84 and the short chain fatty acid receptor FFA2R. Biochim Biophys Acta 1865:695-708 (2018). PubMed: 29477577
- Leprieur EG et al. Membrane-bound full-length Sonic Hedgehog identifies cancer stem cells in human non-small cell lung cancer. Oncotarget 8:103744-103757 (2017). PubMed: 29262597
- Horejsí V et al. Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha) 34:23-34 (1988). PubMed: 2968928