AB272272

APC Anti-CD20抗体[2H7]

Name: APC Anti-CD20抗体[2H7] (ab272272)| Abcam中文官网
Brand: Abcam Limited
SKU: AB272272
Price: 1683 CNY
Availability: InStock

APC Anti-CD20 antibody [2H7]

Be the first to review this product! Submit a review

(1 Publication)

Mouse Monoclonal CD20 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing MS4A1 protein.

查看别名

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

1 Images

Flow Cytometry - APC Anti-CD20 antibody [2H7] (AB272272)

Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD20 antibody [2H7] (AB272272)

Human peripheral blood lymphocytes were stained with FITC Anti-Human CD3 and 5 uL (0.125 ug) ab272272 (right panel) or 0.125 ug APC Mouse IgG2b isotype control (left panel).

不同偶联物与剂型 (9)

785 APC/Cy7®

APC/Cy7® Anti-CD20 antibody [2H7]
723 Alexa Fluor® 700

Alexa Fluor® 700 Anti-CD20 antibody [2H7]
Biotin

Biotin Anti-CD20 antibody [2H7]
519 FITC

FITC Anti-CD20 antibody [2H7]
578 PE

PE Anti-CD20 antibody [2H7]
778 PE/Cy7®

PE/Cy7® Anti-CD20 antibody [2H7]
618 PE/DyLight™ 594

PE/DyLight™ 594 Anti-CD20 antibody [2H7]
675 PerCP

PerCP Anti-CD20 antibody [2H7]
695 PerCP/Cy5.5®

PerCP/Cy5.5® Anti-CD20 antibody [2H7]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

2H7

亚型

IgG2b

轻链类型

kappa

偶联物

APC

激发波长/发射波长

Ex: 650nm, Em: 660nm

不含载体蛋白

反应种属

Human

应用

Flow Cyt

applications

免疫原

Cell preparation containing MS4A1 protein. The exact immunogen used to generate this antibody is proprietary information.

P11836

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>In a volume of 100 μL.</p>" }, "Baboon": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Chimpanzee": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Cynomolgus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rhesus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

纯化说明

Purified from tissue culture supernatant.

存储溶液

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin

运输条件

Blue Ice

储存信息

Store in the dark

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.

Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).

See full target information MS4A1

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 16:220-229 PubMed40116454

2025

-epigenomic reprogramming and maintenance of plasma cell phenotype in t(4;14) myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Gunnell,Scott T Kimber,Richard Houlston,Martin Kaiser

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

APC Anti-CD20抗体[2H7]