AB25482

APC Anti-CD11b抗体[M1/70]

APC Anti-CD11b antibody [M1/70]

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(17 Publications)

Rat Monoclonal CD11b antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 17 publications. Immunogen corresponding to Cell preparation containing Itgam protein.

查看别名

CD11b, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Itgam

1 Images

Flow Cytometry - APC Anti-CD11b antibody [M1/70] (AB25482)

Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD11b antibody [M1/70] (AB25482)

C57Bl/6 bone marrow cells were stained with 0.125μg anti-CD11b (ab25482) (solid line) or 0.125μg APC Rat IgG2b isotype control (dashed line).

不同偶联物与剂型 (15)

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD11b antibody [M1/70]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD11b antibody [M1/70]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD11b antibody [M1/70]
578 PE

PE Anti-CD11b antibody [M1/70]
Unconjugated

Anti-CD11b antibody [M1/70]
Carrier free

Anti-CD11b antibody [M1/70] - BSA and Azide free
778 PE/Cy7®

PE/Cy7® Anti-CD11b antibody [M1/70]
450 violetFluor™ 450

violetFluor™ 450 Anti-CD11b antibody [M1/70]
redFluor™ 710

redFluor™ 710 Anti-CD11b antibody [M1/70]
785 APC/Cy7®

APC/Cy7® Anti-CD11b antibody [M1/70]
Biotin

Biotin Anti-CD11b antibody [M1/70]
578 PE

PE Anti-CD11b antibody [M1/70]
519 FITC

FITC Anti-CD11b antibody [M1/70]
667 PE/Cy5®

PE/Cy5® Anti-CD11b antibody [M1/70]
695 PerCP/Cy5.5®

PerCP/Cy5.5® Anti-CD11b antibody [M1/70]

关键信息

宿主种属

Rat

克隆

Monoclonal

克隆号

M1/70

亚型

IgG2b

轻链类型

kappa

偶联物

APC

激发波长/发射波长

Ex: 650nm, Em: 660nm

不含载体蛋白

反应种属

Mouse

应用

Flow Cyt

applications

免疫原

Cell preparation containing Itgam protein. The exact immunogen used to generate this antibody is proprietary information.

P05555

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }

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产品详情

Protect the product from prolonged exposure to light.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.142% Sodium dihydrogen phosphate, 0.1% Gelatin

运输条件

Blue Ice

储存信息

Do Not Freeze|Store undiluted

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.

Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (By similarity). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (By similarity). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (PubMed : 18685038).

See full target information Itgam

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文献 (17)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40430042

2025

Caveolin-1 Deficiency in Macrophages Alleviates Carbon Tetra-Chloride-Induced Acute Liver Injury in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ruirui Li,Yixue Shu,Yulin Yan,Junyi Zhu,Zilu Cheng,Jie Zhang,Liming Zhu,Yanhua Qiao,Quan Sun

Biology direct 20:61 PubMed40399992

2025

Mechanism of LINC01018/miR-182-5p/Rab27B in the immune escape through PD-L1-mediated CD8 T cell suppression in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Su Hu,Guoshuo Chen,Aiping Luo,Hailin Zhao,Dan Li,Biao Peng,Jike Du,Dongdong Luo

Biology methods & protocols 10:bpaf020 PubMed40177524

2025

A generalized protocol for the induction of M2-like macrophages from mouse and rat bone marrow mononuclear cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ulugbek R Yakhshimurodov,Kizuku Yamashita,Kenji Miki,Takuji Kawamura,Shunsuke Saito,Shigeru Miyagawa

Obesity facts 17:24-36 PubMed37820603

2023

Ube2L6 Promotes M1 Macrophage Polarization in High-Fat Diet-Fed Obese Mice via ISGylation of STAT1 to Trigger STAT1 Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yunqian Li,Xiao Dong,Wenqian He,Huibiao Quan,Kaining Chen,Chaoping Cen,Weiping Wei

Cellular & molecular biology letters 28:79 PubMed37828427

2023

Activation of BZW1 by CEBPB in macrophages promotes eIF2α phosphorylation-mediated metabolic reprogramming and endoplasmic reticulum stress in MRL/lpr lupus-prone mice.

Applications

Unspecified application

Species

Unspecified reactive species

Huimeng Qi,Zhaoguo Zheng,Qiang Liu

Mediators of inflammation 2022:1108569 PubMed36619207

2022

Prevents Ulcerative Colitis by Promoting M2 Macrophage Polarization through Blocking the TLR4/MAPK/NF-B Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Sun,Ping Zhao,Xufeng Ding,Fang Li,Jie Jiang,Hua Huang,Lijiang Ji

EBioMedicine 81:104081 PubMed35660787

2022

Fibrin deposition associates with cartilage degeneration in arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Hügle,Sonia Nasi,Driss Ehirchiou,Patrick Omoumi,Alexander So,Nathalie Busso

Journal of clinical laboratory analysis 36:e24455 PubMed35524480

2022

Dioscin ameliorates inflammatory bowel disease by up-regulating miR-125a-5p to regulate macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyan Shi,Peichen Zhang,Ruifang Jin,Xiaowei Chen,Lemei Dong,Weichang Chen

PloS one 15:e0228889 PubMed32045450

2020

Mesenchymal stem cells reduce alcoholic hepatitis in mice via suppression of hepatic neutrophil and macrophage infiltration, and of oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yue-Meng Wan,Zhi-Qiang Li,Chang Liu,Yue-Feng He,Men-Jie Wang,Xi-Nan Wu,Yuan Zhang,Yu-Hua Li

Frontiers in immunology 10:2969 PubMed31969881

2020

FA-97, a New Synthetic Caffeic Acid Phenethyl Ester Derivative, Ameliorates DSS-Induced Colitis Against Oxidative Stress by Activating Nrf2/HO-1 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Mei,Zihao Wang,Yifan Zhang,Ting Wan,Jincheng Xue,Wei He,Yi Luo,Yijun Xu,Xue Bai,Qi Wang,Yujie Huang

View all publications

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