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AB278785

重组APC Anti-ASK1 (phospho T838)抗体[Ask1T838-8D12]

APC Anti-ASK1 (phospho T838) antibody [Ask1T838-8D12]

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(1 Publication)

Rabbit Recombinant Monoclonal ASK1 phospho T838 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MAP3K5 pT838.

查看别名

ASK1, MAPKKK5, MEKK5, MAP3K5, Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, MAPK/ERK kinase kinase 5, ASK-1, MEK kinase 5, MEKK 5

1 Images
Flow Cytometry - APC Anti-ASK1 (phospho T838) antibody [Ask1T838-8D12] (AB278785)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-ASK1 (phospho T838) antibody [Ask1T838-8D12] (AB278785)

Flow cytometric analysis of NIH3T3 cells treated with staurosporine and unstained as negative control (blue) or treated with staurosporine (red) or with PDGF (green) and stained using ab278785.

不同偶联物与剂型 (1)

  • Unconjugated

    Anti-ASK1 (phospho T838) antibody [Ask1T838-8D12]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

Ask1T838-8D12

亚型

IgG

轻链类型

kappa

偶联物

APC

激发波长/发射波长

Ex: 650nm, Em: 660nm

不含载体蛋白

No

反应种属

Mouse

应用

Flow Cyt

applications

免疫原

Synthetic Peptide within Human MAP3K5 pT838. The exact immunogen used to generate this antibody is proprietary information.

Q99683

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A/G
存储溶液
Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The ASK1 protein also known as apoptosis signal-regulating kinase 1 is an important component in cellular stress response pathways. This 150 kDa protein is a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family. ASK1 is expressed in several tissues including the heart brain liver and pancreas. Mechanically ASK1 gets activated in response to a variety of stress stimuli such as oxidative stress and endoplasmic reticulum stress which significantly impact the regulation of programmed cell death.
Biological function summary

In response to stressors ASK1 activates the c-Jun N-terminal kinase (JNK) and p38 MAPK signaling pathways which play roles in apoptosis and inflammation. ASK1 tends to form a complex with TRAF2 and TRAF6 key adapters in downstream signaling. This kinase acts as an important link bridging environmental stress signals to cellular responses. Through its function ASK1 influences cell fate decisions in response to various stress conditions.

Pathways

ASK1 is a central player in the MAPK signaling cascade. Within this cascade ASK1 regulates JNK and p38 MAPK pathways which are significant in stress adaptation and cellular responses. ASK1 interacts with proteins like MKK4 and MKK7 in the JNK pathway amplifying the stress signals. Through these interactions ASK1 ensures the propagation of signals important for adaptive responses or programmed cell death.

Dysfunction in ASK1 activity associates with neurodegenerative diseases and cardiovascular disorders. In Alzheimer's disease for instance ASK1 contributes to neuronal apoptosis via its engagement with JNK signaling. In cardiovascular disorders ASK1 exacerbates heart failure by promoting cell death and inflammation. ASK1's interaction with proteins such as TNF receptor-associated factor 2 (TRAF2) highlights its role in these pathological conditions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).
See full target information MAP3K5 pT838
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 54:2162-2174 PubMed36473935

2022

ASK1-ER stress pathway-mediated fibrotic-EV release contributes to the interaction of alveolar epithelial cells and lung fibroblasts to promote mechanical ventilation-induced pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ri Tang,Shuya Mei,Qiaoyi Xu,Jinhua Feng,Yang Zhou,Shunpeng Xing,Zhengyu He,Yuan Gao
View all publications
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