重组APC Anti-Annexin A1/ANXA1抗体[EPR19342] (ab225511)
![Flow Cytometry (Intracellular) - APC Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225511)](https://www.abcam.cn/ps/products/225/ab225511/Images/ab225511-312642-anti-annexin-a1-antibody-epr19342-allophycocyanin-flow-cytometry.jpg "Flow Cytometry (Intracellular) - APC Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225511)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EPR19342] to Annexin A1/ANXA1
- Suitable for: Flow Cyt (Intra)
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
Related conjugates and formulations
概述
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产品名称
APC Anti-Annexin A1/ANXA1抗体[EPR19342]
参阅全部 Annexin A1/ANXA1 一抗 -
描述
APC兔单克隆抗体[EPR19342] to Annexin A1/ANXA1 -
偶联物
APC. Ex: 645nm, Em: 660nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Mouse
预测可用于: Rat, Human
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免疫原
Recombinant fragment within Human Annexin A1/ANXA1 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P04083 -
阳性对照
- Flow Cyt (intra): NIH/3T3 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19342 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab214486)
- Anti-Annexin A1/ANXA1 antibody [EPR19342] - BSA and Azide free (ab222398)
- PE Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225512)
- Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225513)
- Alexa Fluor® 647 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab309365)
- Alexa Fluor® 555 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab313664)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225511于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/5000.
The cellular localisation of this product has been verified in ICC/IF. |
| 说明 |
|---|
|
Flow Cyt (Intra)
1/5000. The cellular localisation of this product has been verified in ICC/IF. |
靶标
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功能
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. -
序列相似性
Belongs to the annexin family.
Contains 4 annexin repeats. -
结构域
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
翻译后修饰
Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity. -
细胞定位
Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages. - Information by UniProt
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数据库链接
- Entrez Gene: 301 Human
- Entrez Gene: 16952 Mouse
- Entrez Gene: 25380 Rat
- Omim: 151690 Human
- SwissProt: P04083 Human
- SwissProt: P10107 Mouse
- SwissProt: P07150 Rat
- Unigene: 494173 Human
see all -
别名
- Annexin 1 antibody
- Annexin A1 antibody
- Annexin I (lipocortin I) antibody
see all
图片
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Flow Cytometry (Intracellular) - APC Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225511)
Overlay histogram showing NIH/3T3 cells stained with ab225511 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225511, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (ab232814) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
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![APC Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225511)](https://www.abcam.cn/ps/products/225/ab225511/Images/ab225511-3-benefits-of-recombinant-antibodies.png)
APC Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225511)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab225511 尚未被引用在任何文献中。
