重组APC Anti-6X His tag®抗体[EPR20547] - ChIP Grade (ab317659)
![Immunocytochemistry/ Immunofluorescence - APC Anti-6X His tag® antibody [EPR20547] (ab317659)](https://www.abcam.cn/ps/products/317/ab317659/Images/ab317659-2-apc-anti-6x-his-tag-antibody-epr20547-immunocytochemistry-293t.jpg "Immunocytochemistry/ Immunofluorescence - APC Anti-6X His tag® antibody [EPR20547] (ab317659)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EPR20547] to 6X His tag® - ChIP Grade
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Species independent
- Conjugation: APC. Ex: 645nm, Em: 660nm
Related conjugates and formulations
概述
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产品名称
APC Anti-6X His tag®抗体[EPR20547] - ChIP Grade
参阅全部 6X His tag® 一抗 -
描述
APC兔单克隆抗体[EPR20547] to 6X His tag® - ChIP Grade -
宿主
Rabbit -
偶联物
APC. Ex: 645nm, Em: 660nm -
经测试应用
适用于: Flow Cyt (Intra), ICC/IFmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (Intra): 293T cells transfected with a mouse C1QA expression vector containing a Myc tag. ICC/IF: 293T cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20547 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204)
- Anti-6X His tag® antibody [EPR20547] - BSA and Azide free (ab232492)
- Alexa Fluor® 488 Anti-6X His tag® antibody [EPR20547] (ab237336)
- Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (ab237337)
- PE Anti-6X His tag® antibody [EPR20547] (ab237338)
- HRP Anti-6X His tag® antibody [EPR20547] (ab237339)
- Biotin Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab316173)
- FITC Anti-6X His tag® [EPR20547] (ab318311)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317659于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/5000.
|
|
| ICC/IF |
1/50.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/5000. |
|
ICC/IF
1/50. |
靶标
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相关性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
细胞定位
Depends upon the localization of the parent protein tagged with hexahistidine. -
别名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - APC Anti-6X His tag® antibody [EPR20547] (ab317659)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling 6X His tag with ab317659 at 1/50 (10.0 ug/ml) dilution (Magenta).
Confocal image showing positive staining in 293T cells transfected with a myc-his tag (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Green). The Nuclear counterstain was DAPI (Blue).
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![Flow Cytometry (Intracellular) - APC Anti-6X His tag® antibody [EPR20547] (ab317659)](https://www.abcam.cn/ps/products/317/ab317659/Images/ab317659-3-apc-anti-6x-his-tag-antibody-epr20547-flow-cytometry-isotype.jpg)
Flow Cytometry (Intracellular) - APC Anti-6X His tag® antibody [EPR20547] (ab317659)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T (human embryonic kidney epithelial cell) cells transfected with a mouse C1QA expression vector containing a Myc tag(Middle) / 293T cells containing a Myc tag (Right) cells labelling 6X His tag with ab317659 at 1/5000 dilution(0.01ug) / Middle and Right (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
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![APC Anti-6X His tag® antibody [EPR20547] (ab317659)](https://www.abcam.cn/ps/products/317/ab317659/Images/ab317659-1-BenefitsofRecombinantantibodies.jpg)
APC Anti-6X His tag® antibody [EPR20547] (ab317659)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317659 尚未被引用在任何文献中。