Anti-APAF1抗体(ab46899)
Key features and details
- Rabbit polyclonal to APAF1
- Suitable for: IHC-FoFr
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-APAF1抗体
参阅全部 APAF1 一抗 -
描述
兔多克隆抗体to APAF1 -
宿主
Rabbit -
经测试应用
适用于: IHC-FoFrmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human APAF1 aa 1200 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: O14727 -
阳性对照
- Testis
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab46899于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-FoFr |
1/200.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
说明 |
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IHC-FoFr
1/200. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
靶标
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功能
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. -
组织特异性
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. -
序列相似性
Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats. -
结构域
The CARD domain mediates interaction with APIP. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 317 Human
- Entrez Gene: 11783 Mouse
- Entrez Gene: 78963 Rat
- Omim: 602233 Human
- SwissProt: O14727 Human
- SwissProt: O88879 Mouse
- SwissProt: Q9EPV5 Rat
- Unigene: 728891 Human
see all -
别名
- APAF 1 antibody
- Apaf-1 antibody
- APAF_HUMAN antibody
see all
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab46899 尚未被引用在任何文献中。