AB128950

重组Anti-AP2S1抗体[EPR2697(2)]

Name: AP2S1抗体[EPR2697(2)] (ab128950)| Abcam中文官网
Brand: Abcam Limited
SKU: AB128950
Price: 2347 CNY
Availability: InStock
Rating: 2 (1 reviews)

Anti-AP2S1 antibody [EPR2697(2)]

RabMAb
Recombinant
了解详情

(1 Review)

(15 Publications)

Rabbit Recombinant Monoclonal AP2S1 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 15 publications.

查看别名

AP17, CLAPS2, AP2S1, AP-2 complex subunit sigma, Adaptor protein complex AP-2 subunit sigma, Adaptor-related protein complex 2 subunit sigma, Clathrin assembly protein 2 sigma small chain, Clathrin coat assembly protein AP17, Clathrin coat-associated protein AP17, HA2 17 kDa subunit, Plasma membrane adaptor AP-2 17 kDa protein, Sigma2-adaptin

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling AP2S1 with purified ab128950 at 1 : 100 dilution (12.07 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

ab128950, at a 1/250 dilution, staining AP2S1 in paraffin embedded Human colon carcinoma tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling AP2S1 with purified ab128950 at 1 : 100 dilution (12.07 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

ab128950, at a 1/250 dilution, staining AP2S1 in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

Unknown

Immunoprecipitation - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

ab128950 (purified) at 1 : 60 dilution (2μg) immunoprecipitating AP2S1 in Human fetal brain lysate.
Lane 1 (input) : Human fetal brain lysate 10μg
Lane 2 (+) : ab128950 & Human fetal brain lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab128950 in Human fetal brain lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)

Predicted band size: 17 kDa

false

Unknown

Western blot - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

All lanes:

Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

SH-SY5Y lysate at 10 µg

Lane 3:

293T lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 17 kDa

false

Lab

Western blot - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

14% gel is preferred to 4-20% gradient gel

All lanes:

Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950) at 1/1000 dilution

Lane 1:

Human fetal brain lysates at 15 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 4:

Mouse brain lysates at 15 µg

Lane 5:

Mouse kidney lysates at 15 µg

Lane 6:

Rat brain lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Lab

Western blot - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

All lanes:

Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950) at 1/1000 dilution

All lanes:

Mouse kidney lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Lab

Western blot - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

All lanes:

Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

Mouse brain lysates at 15 µg

Lane 3:

Rat brain lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR2697(2)

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IHC-P, IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.For unpurified use at 1/250 - 1/500." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

AP2S1 also known as adaptor-related protein complex 2 sigma 1 subunit is a protein with an estimated molecular mass of approximately 20 kDa. It is expressed in various human tissues but shows higher expression in the brain. This protein is a component of the adaptor protein 2 (AP-2) complex which plays an important role in clathrin-mediated endocytosis a process essential for the internalization of various molecules into the cell.

Biological function summary

The AP2S1 protein functions as part of the AP-2 complex which regulates the recruitment of clathrin to the plasma membrane facilitating the formation of clathrin-coated vesicles. These vesicles help in the transport of transferrin and its receptor and also influence the internalization of membrane proteins and lipids. This efficient transport system supports cellular activities like receptor recycling and nutrient uptake making it an important participant in cellular homeostasis.

Pathways

AP2S1 is involved in endocytic and vesicular transport pathways. It plays a role in the endocytic trafficking pathway that includes the involvement of other proteins like clathrin and AP2B1 which work together to manage cellular intake of nutrients and receptor-ligand complexes. The AP-2 complex functions as a bridge between cargo proteins and the clathrin lattice during the formation of transport vesicles controlling vital cellular processes.

AP2S1 has implications in conditions like familial hypocalciuric hypercalcemia type 3 (FHH3) and Brooks-Widemann Syndrome. Mutations in the AP2S1 gene disrupt the normal calcium-sensing receptor pathway which leads to altered calcium homeostasis manifesting as hypercalcemia in patients with FHH3. This condition links AP2S1 functionally with proteins involved in calcium regulation highlighting its significance in maintaining cellular and systemic equilibrium.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity). May also play a role in extracellular calcium homeostasis.

See full target information AP2S1

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文献 (15)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadw9158 PubMed40938986

2025

Bi-handed assembly chaperones regulate protein complex assembly through an intramolecular handover mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Wu,Chun Wan,Yuan Tian,Yan Ouyang,Harrison Puscher,Suzhao Li,Qian Yin,Jingshi Shen

Nature communications 16:2254 PubMed40050266

2025

The essential clathrin adapter protein complex-2 is tumor suppressive specifically in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Seth P Zimmerman,Lili B DeGraw,Christopher M Counter

Proceedings of the National Academy of Sciences of the United States of America 121:e2409341121 PubMed39145939

2024

An AAGAB-to-CCDC32 handover mechanism controls the assembly of the AP2 adaptor complex.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Wan,Harrison Puscher,Yan Ouyang,Jingyi Wu,Yuan Tian,Suzhao Li,Qian Yin,Jingshi Shen

The Biochemical journal 481:1143-1171 PubMed39145956

2024

CARD14 signalosome formation is associated with its endosomal relocation and mTORC1-induced keratinocyte proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Paul A O'Sullivan,Aigerim Aidarova,Inna S Afonina,Joan Manils,Teresa L M Thurston,Rachael Instrell,Michael Howell,Stefan Boeing,Sashini Ranawana,Melanie B Herpels,Riwia Chetian,Matilda Bassa,Helen Flynn,David Frith,Ambrosius P Snijders,Ashleigh Howes,Rudi Beyaert,Anne M Bowcock,Steven C Ley

Virulence 15:2350893 PubMed38725096

2024

effector CvpE maintains biogenesis of -containing vacuoles by suppressing lysosome tubulation through binding PI(3)P and perturbing PIKfyve activity on lysosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Mingliang Zhao,Shan Zhang,Weiqiang Wan,Chunyu Zhou,Nana Li,Ruxi Cheng,Yonghui Yu,Xuan Ouyang,Dongsheng Zhou,Jun Jiao,Xiaolu Xiong

Journal of pineal research 75:e12897 PubMed37391878

2023

Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin.

Applications

Unspecified application

Species

Unspecified reactive species

Jingming Ren,Tong Zhu,Zhouxuan Wang,Lu Lu,Saijun Fan

Traffic (Copenhagen, Denmark) 24:20-33 PubMed36412210

2022

AP2S1 regulates APP degradation through late endosome-lysosome fusion in cells and APP/PS1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Xin Wen,Biao Luo,Xiao-Yong Xie,Gui-Feng Zhou,Jian Chen,Li Song,Yue Liu,Shi-Qi Xie,Long Chen,Kun-Yi Li,Xiao-Jiao Xiang,Guo-Jun Chen

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed34155137

2021

CALM supports clathrin-coated vesicle completion upon membrane tension increase.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan M Willy,Federico Colombo,Scott Huber,Anna C Smith,Erienne G Norton,Comert Kural,Emanuele Cocucci

Traffic (Copenhagen, Denmark) 21:603-616 PubMed32657003

2020

An internally eGFP-tagged α-adaptin is a fully functional and improved fiduciary marker for clathrin-coated pit dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Rosa E Mino,Zhiming Chen,Marcel Mettlen,Sandra L Schmid

Neurobiology of disease 141:104948 PubMed32434048

2020

Sequential screening nominates the Parkinson's disease associated kinase LRRK2 as a regulator of Clathrin-mediated endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

George R Heaton,Natalie Landeck,Adamantios Mamais,Mike A Nalls,Jonathon Nixon-Abell,Ravindran Kumaran,Alexandra Beilina,Laura Pellegrini,Yan Li,Kirsten Harvey,Mark R Cookson

View all publications

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重组Anti-AP2S1抗体[EPR2697(2)]

Anti-AP2S1 antibody [EPR2697(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

Immunoprecipitation - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

Western blot - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

Western blot - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

Western blot - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

Western blot - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)