AB129168

Anti-AP2B1 抗体 [EPR7567]

Anti-AP2B1 antibody [EPR7567]

RabMAb
Recombinant
了解详情

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(4 Publications)

Rabbit Recombinant Monoclonal AP2B1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

查看别名

ADTB2, CLAPB1, AP2B1, AP-2 complex subunit beta, AP105B, Adaptor protein complex AP-2 subunit beta, Adaptor-related protein complex 2 subunit beta, Beta-2-adaptin, Beta-adaptin, Clathrin assembly protein complex 2 beta large chain, Plasma membrane adaptor HA2/AP2 adaptin beta subunit

2 Images

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Western blot - Anti-AP2B1 antibody [EPR7567] (AB129168)

All lanes:

Western blot - Anti-AP2B1 antibody [EPR7567] (ab129168) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 105 kDa

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OI-RD Scanning - Anti-AP2B1 antibody [EPR7567] (AB129168)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-AP2B1 antibody [EPR7567] (AB129168)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (1)

Carrier free

Anti-AP2B1 antibody [EPR7567] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR7567

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

AP2B1 also known as the beta-1 subunit of the adaptor protein complex 2 (AP-2 complex) has a mass of approximately 105 kDa. It plays an important role in clathrin-mediated endocytosis by forming a bridge between clathrin and cargo molecules. The AP-2 complex is essential for selecting and concentrating cargo proteins that are destined for endocytosis. AP2B1 is highly expressed in most tissues with abundant presence in brain tissue suggesting its importance in neural processes.

Biological function summary

AP2B1 functions as a component of the AP-2 complex which is essential for the formation of clathrin-coated pits on the plasma membrane. It contributes to the regulation of receptor-mediated endocytosis a process important for nutrient uptake and signaling receptor regulation. The AP-2 complex binds to the cytoplasmic tails of transmembrane proteins mediating their internalization into cells for degradation or recycling. This highlights AP2B1's role in maintaining cellular homeostasis and responding to environmental signals.

Pathways

Researchers have shown that AP2B1 is actively involved in the endocytic pathway facilitating the internalization of cell surface receptors into vesicles. It is also linked to the sorting of cargo proteins within the endocytosis pathway in tandem with other proteins like calmodulin. The interaction with dynamin regulates the membrane scission events necessary for vesicle release. Both calmodulin and dynamin are important for the successful execution of these pathways where AP2B1 plays a central role.

Disruptions in AP2B1 function link to neurological disorders such as Alzheimer’s disease potentially due to impaired endocytosis and subsequent cellular signaling dysfunctions. Abnormalities in the AP-2 complex including AP2B1 have also been associated with cancers like melanoma where altered receptor endocytosis can lead to uncontrolled cell signaling and proliferation. Both conditions involve pathways where dynamin works closely with AP2B1 hinting at its wider implications in disease progression and therapeutic target potential.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed : 23676497). The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly.

See full target information AP2B1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:226 PubMed40544244

2025

Prognostic and therapeutic potential of disulfidptosis-related genes in colon adenocarcinoma: a comprehensive multi-omics study.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Song,Haoran Zhu,Junyang Wei,Shanxue Yin

Genes to cells : devoted to molecular & cellular mechanisms 29:1154-1172 PubMed39377417

2024

The scaffold protein IQGAP1 promotes reorientation of epithelial cell polarity at the two-cell stage for cystogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Michihiro Horikawa,Junya Hayase,Sachiko Kamakura,Akira Kohda,Masafumi Nakamura,Hideki Sumimoto

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed34155137

2021

CALM supports clathrin-coated vesicle completion upon membrane tension increase.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan M Willy,Federico Colombo,Scott Huber,Anna C Smith,Erienne G Norton,Comert Kural,Emanuele Cocucci

Neurobiology of disease 141:104948 PubMed32434048

2020

Sequential screening nominates the Parkinson's disease associated kinase LRRK2 as a regulator of Clathrin-mediated endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

George R Heaton,Natalie Landeck,Adamantios Mamais,Mike A Nalls,Jonathon Nixon-Abell,Ravindran Kumaran,Alexandra Beilina,Laura Pellegrini,Yan Li,Kirsten Harvey,Mark R Cookson

View all publications

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Anti-AP2B1 抗体 [EPR7567]