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AB151961

Anti-AP2B1 抗体

Anti-AP2B1 antibody

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(1 Publication)

Rabbit Polyclonal AP2B1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AP2B1 aa 300 to C-terminus.

查看别名

ADTB2, CLAPB1, AP2B1, AP-2 complex subunit beta, AP105B, Adaptor protein complex AP-2 subunit beta, Adaptor-related protein complex 2 subunit beta, Beta-2-adaptin, Beta-adaptin, Clathrin assembly protein complex 2 beta large chain, Plasma membrane adaptor HA2/AP2 adaptin beta subunit

3 Images
Western blot - Anti-AP2B1 antibody (AB151961)
  • WB

Unknown

Western blot - Anti-AP2B1 antibody (AB151961)

7.5% SDS PAGE

All lanes:

Western blot - Anti-AP2B1 antibody (ab151961) at 1/1000 dilution

All lanes:

IMR32 whole cell lysate at 30 µg

Predicted band size: 105 kDa

false

Western blot - Anti-AP2B1 antibody (AB151961)
  • WB

Unknown

Western blot - Anti-AP2B1 antibody (AB151961)

7.5% SDS PAGE

All lanes:

Western blot - Anti-AP2B1 antibody (ab151961) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 105 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2B1 antibody (AB151961)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2B1 antibody (AB151961)

Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labeling AP2B1 with ab151961 antibody at 1/250.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human AP2B1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P63010

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100.00000 - 1/1000.00000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500.00000 - 1/3000.00000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500.00000 - 1/3000.00000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

AP2B1 also known as the beta-1 subunit of the adaptor protein complex 2 (AP-2 complex) has a mass of approximately 105 kDa. It plays an important role in clathrin-mediated endocytosis by forming a bridge between clathrin and cargo molecules. The AP-2 complex is essential for selecting and concentrating cargo proteins that are destined for endocytosis. AP2B1 is highly expressed in most tissues with abundant presence in brain tissue suggesting its importance in neural processes.
Biological function summary

AP2B1 functions as a component of the AP-2 complex which is essential for the formation of clathrin-coated pits on the plasma membrane. It contributes to the regulation of receptor-mediated endocytosis a process important for nutrient uptake and signaling receptor regulation. The AP-2 complex binds to the cytoplasmic tails of transmembrane proteins mediating their internalization into cells for degradation or recycling. This highlights AP2B1's role in maintaining cellular homeostasis and responding to environmental signals.

Pathways

Researchers have shown that AP2B1 is actively involved in the endocytic pathway facilitating the internalization of cell surface receptors into vesicles. It is also linked to the sorting of cargo proteins within the endocytosis pathway in tandem with other proteins like calmodulin. The interaction with dynamin regulates the membrane scission events necessary for vesicle release. Both calmodulin and dynamin are important for the successful execution of these pathways where AP2B1 plays a central role.

Disruptions in AP2B1 function link to neurological disorders such as Alzheimer’s disease potentially due to impaired endocytosis and subsequent cellular signaling dysfunctions. Abnormalities in the AP-2 complex including AP2B1 have also been associated with cancers like melanoma where altered receptor endocytosis can lead to uncontrolled cell signaling and proliferation. Both conditions involve pathways where dynamin works closely with AP2B1 hinting at its wider implications in disease progression and therapeutic target potential.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed : 23676497). The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly.
See full target information AP2B1
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 7: PubMed29345618

2018

NECAPs are negative regulators of the AP2 clathrin adaptor complex.

Applications

Unspecified application

Species

Unspecified reactive species

Gwendolyn M Beacham,Edward A Partlow,Jeffrey J Lange,Gunther Hollopeter
View all publications
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