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AB61

Anti-AP2 alpha + beta抗体[A6/2/2]

Anti-AP2 alpha + beta antibody [A6/2/2]

4

(1 Review)

|

(3 Publications)

Mouse Monoclonal Transcription factor AP-2-alpha antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TFAP2A aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

查看别名

AP2TF, TFAP2, TFAP2A, Transcription factor AP-2-alpha, AP2-alpha, AP-2 transcription factor, Activating enhancer-binding protein 2-alpha, Activator protein 2, AP-2

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-AP2 alpha + beta antibody [A6/2/2] (AB61)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AP2 alpha + beta antibody [A6/2/2] (AB61)

ICC/IF image of ab61 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

A6/2/2

亚型

IgG1

轻链类型

unknown

不含载体蛋白

No

反应种属

Human

应用

ICC/IF

applications

免疫原

Synthetic Peptide within Human TFAP2A aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P05549

特异性

Reacts with both AP-2 alpha and beta forms. As the antibody also recognises AP2 beta, the epitope it recognises is probably the last 8 amino acids as these are common between alpha and beta.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Preservative: 0.02% Sodium azide Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

AP2 alpha + beta also known as adaptor protein complex 2 functions as part of the protein coat complex that facilitates clathrin-mediated endocytosis. Composed of alpha and beta subunits this complex has an approximate mass of 200 kDa. You can find AP2 alpha + beta highly expressed in cells with active endocytic processes such as neurons and epithelial cells.
Biological function summary

AP2 alpha + beta mediates the recruitment and assembly of clathrin-coated vesicles at the plasma membrane. This complex interacts directly with clathrin and cargo receptors playing an important role in cargo selection during endocytosis. It has an important role in cellular nutrient uptake and receptor internalization which are necessary for maintaining cellular function and signaling.

Pathways

Functions of AP2 alpha + beta are integral to the endocytic pathway and intracellular trafficking. It interacts with proteins such as clathrin and dynamin to ensure proper vesicle maturation and scission. You can find it involved in pathways that regulate cell surface receptor levels and synaptic vesicle recycling highlighting its interaction with proteins like AP180 and Eps15.

AP2 alpha + beta involvement correlates with neurological disorders and cancer. Abnormal function or expression of these proteins has been observed in Alzheimer’s disease where altered endocytosis affects amyloid precursor protein processing. Additionally AP2 alpha + beta mutations or dysregulation has connections to tumor progression often linked with oncogenic receptor endocytosis.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.
See full target information TFAP2A

其他靶点

TFAP2B
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 4:101309 PubMed38086379

2023

Econazole selectively induces cell death in NF1-homozygous mutant tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yenal B Lakes,Stefanie L Moye,Juan Mo,Matthew Tegtmeyer,Ralda Nehme,Maura Charlton,Gabrielle Salinas,Renee M McKay,Kevin Eggan,Lu Q Le

Genome biology 21:152 PubMed32580738

2020

Single-cell transcriptome and antigen-immunoglobin analysis reveals the diversity of B cells in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Chen,Yun Tan,Fenghuan Sun,Likun Hou,Chi Zhang,Tao Ge,Huansha Yu,Chunxiao Wu,Yuming Zhu,Liang Duan,Liang Wu,Nan Song,Liping Zhang,Wei Zhang,Di Wang,Chang Chen,Chunyan Wu,Gening Jiang,Peng Zhang

The Journal of pathology 189:514-20 PubMed10629551

2000

Immunohistochemical analysis reveals a tumour suppressor-like role for the transcription factor AP-2 in invasive breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

J M Gee,J F Robertson,I O Ellis,R I Nicholson,H C Hurst
View all publications
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