AB249235

重组Anti-AP-A抗体[EPR9411(2)] - BSA and Azide free

Anti-AP-A antibody [EPR9411(2)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal AP-A antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

查看别名

CD249, Glutamyl aminopeptidase, EAP, Aminopeptidase A, Differentiation antigen gp160, AP-A, ENPEP

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] - BSA and Azide free (AB249235)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] - BSA and Azide free (AB249235)

This data was developed using ab155991, the same antibody clone in a different buffer formulation.

ab155991 showing +ve staining in Human vascular endothelium.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] - BSA and Azide free (AB249235)

This data was developed using ab155991, the same antibody clone in a different buffer formulation.

ab155991 showing -ve staining in Human normal prostate.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] - BSA and Azide free (AB249235)

This data was developed using ab155991, the same antibody clone in a different buffer formulation.

ab155991 showing -ve staining in Human ovarian carcinoma.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] - BSA and Azide free (AB249235)

This data was developed using ab155991, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling AP-A with ab155991 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] - BSA and Azide free (AB249235)

This data was developed using ab155991, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling AP-A with ab155991 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] - BSA and Azide free (AB249235)

This data was developed using ab155991, the same antibody clone in a different buffer formulation.

ab155991 showing -ve staining in Human normal tonsil.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-AP-A antibody [EPR9411(2)] - BSA and Azide free (AB249235)

This data was developed using ab155991, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AP-A antibody [EPR9411(2)] (<a href='/products/primary-antibodies/ap-a-antibody-epr94112-ab155991'>ab155991</a>) at 1/1000 dilution

Lane 1:

Human fetal lung lysate at 10 µg

Lane 2:

Human fetal kidney lysates at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 109 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-AP-A antibody [EPR9411(2)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR9411(2)

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

ab249235 is the carrier-free version of ab155991.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

AP-A also known as Aminopeptidase A is an enzyme that plays a critical role in cellular processes by cleaving N-terminal amino acids from peptide substrates. It has a molecular mass of approximately 100 kDa. AP-A is highly expressed in the brain particularly in the hippocampus and glial cells as well as in other tissues like the kidney and placenta. Its activity has been observed in the synaptic membranes signifying its importance in neurobiological functions.

Biological function summary

Aminopeptidase A participates in the regulation of neuropeptides and peptide hormones. It forms part of the renin-angiotensin system where it influences blood pressure and fluid balance. By converting angiotensin II to angiotensin III AP-A modulates these physiological processes. Moreover it is involved in degrading enkephalins which impacts pain perception and emotional responses. This enzyme interacts with other aminopeptidases in the body contributing to various cellular functions.

Pathways

AP-A significantly impacts the renin-angiotensin system and the endothelin pathway. It influences the cardiovascular system by processing angiotensin peptides working closely with proteins such as angiotensin-converting enzyme (ACE). In the endothelin pathway AP-A affects peptide regulation and is associated with the activity of endothelin-converting enzyme. These pathways demonstrate the enzyme's role in cardiovascular health and homeostasis.

Aminopeptidase A is linked to hypertension and neurological disorders like Alzheimer's disease. Increased activity or expression of AP-A is often seen in the context of high blood pressure where it works in conjunction with ACE to modulate blood pressure levels. In Alzheimer's disease altered AP-A activity affects neuropeptide regulation linking it to beta-amyloid accumulation. These associations highlight the enzyme's potential as a target for therapeutic interventions in hypertension and neurodegenerative diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II.

See full target information ENPEP

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-AP-A抗体[EPR9411(2)] - BSA and Azide free