重组Anti-Anterior Gradient 2抗体[EPR3278] (ab76473)
![Western blot - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.cn/ps/products/76/ab76473/Images/ab76473-91912-anti-anterior-gradient-2-antibody-epr3278-western-blot.jpg "Western blot - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3278] to Anterior Gradient 2
- Suitable for: WB, IP, IHC-P, Flow Cyt (Intra), ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Anterior Gradient 2抗体[EPR3278]
参阅全部 Anterior Gradient 2 一抗 -
描述
兔单克隆抗体[EPR3278] to Anterior Gradient 2 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-P, Flow Cyt (Intra), ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Anterior Gradient 2 aa 1-100. The exact sequence is proprietary.
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阳性对照
- HT-29 and MCF-7 cell lysates. Human colon tissue. Human endometrium adenocarcinoma. ICC/IF: MCF7 cells Flow Cyt (intra): HT-29 cells IP: HT-29 whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3278 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Anterior Gradient 2 antibody [EPR3278] - BSA and Azide free (ab181829)
- Alexa Fluor® 488 Anti-Anterior Gradient 2 antibody [EPR3278] (ab199044)
- Alexa Fluor® 555 Anti-Anterior Gradient 2 antibody [EPR3278] (ab215293)
- Alexa Fluor® 568 Anti-Anterior Gradient 2 antibody [EPR3278] (ab312611)
- Alexa Fluor® 750 Anti-Anterior Gradient 2 antibody [EPR3278] (ab321729)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76473于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/5000 - 1/10000. Predicted molecular weight: 20 kDa.
|
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| IP |
1/20.
|
|
| IHC-P | (1) |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Flow Cyt (Intra) |
1/40.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF |
1/100.
|
| 说明 |
|---|
|
WB
1/5000 - 1/10000. Predicted molecular weight: 20 kDa. |
|
IP
1/20. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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Flow Cyt (Intra)
1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
1/100. |
靶标
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功能
Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. -
组织特异性
Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines. -
序列相似性
Belongs to the AGR family. -
细胞定位
Secreted. Endoplasmic reticulum. - Information by UniProt
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数据库链接
- Entrez Gene: 10551 Human
- Omim: 606358 Human
- SwissProt: O95994 Human
- Unigene: 530009 Human
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别名
- AG 2 antibody
- AG 2 protein antibody
- AG-2 antibody
see all
图片
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Western blot - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)All lanes : Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473) at 1/10000 dilution
Lane 1 : HT-29 cell lysates
Lane 2 : MCF-7 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 20 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.cn/ps/products/76/ab76473/Images/ab76473-4-anti-anterior-gradient-2-antibody-epr3278-immunocytochemistry-mcf7-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling Anterior Gradient 2 with purified ab76473 at 1/100 dilution (3.5 µg/mL). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.cn/ps/products/76/ab76473/Images/ab76473-91911-anti-anterior-gradient-2-antibody-epr3278-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.cn/ps/products/76/ab76473/Images/ab76473-7-anti-anterior-gradient-2-antibody-epr3278-immunoprecipitation-ht29-human.jpg)
Immunoprecipitation - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)Purified ab76473 at 1/20 dilution (2µg) immunoprecipitating Anterior Gradient 2 in HT-29 whole cell lysate.
Lane 1 (input): HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab76473 + HT-29 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76473 in HT-29 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 17 kDa -
![Flow Cytometry (Intracellular) - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.cn/ps/products/76/ab76473/Images/ab76473-6-anti-anterior-gradient-2-antibody-epr3278-flowcytometry-ht29-human.jpg)
Flow Cytometry (Intracellular) - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)Intracellular Flow Cytometry analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling Anterior Gradient 2 with purified ab76473 at 1/40 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.cn/ps/products/76/ab76473/Images/ab76473-91910-anti-anterior-gradient-2-antibody-epr3278-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human endometrium adenocarcinoma by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.cn/ps/products/76/ab76473/Images/ab76473-5-benefits-of-recombinant-antibodies.png)
Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab76473 被引用在 21 文献中.
- Lu T et al. Bioinformatics analysis and single-cell RNA sequencing: elucidating the ubiquitination pathways and key enzymes in lung adenocarcinoma. J Thorac Dis 15:3885-3907 (2023). PubMed: 37559628
- Takada H et al. Single-cell transcriptomics uncovers EGFR signaling-mediated gastric progenitor cell differentiation in stomach homeostasis. Nat Commun 14:3750 (2023). PubMed: 37386010
- Wu D et al. Glutamine promotes O-GlcNAcylation of G6PD and inhibits AGR2 S-glutathionylation to maintain the intestinal mucus barrier in burned septic mice. Redox Biol 59:102581 (2023). PubMed: 36565645
- Zhou M et al. AGR2 and FOXA1 as prognostic markers in ER-positive breast cancer. BMC Cancer 23:743 (2023). PubMed: 37568077
- Ly TTG et al. Inhibitory Effect of Etravirine, a Non-Nucleoside Reverse Transcriptase Inhibitor, via Anterior Gradient Protein 2 Homolog Degradation against Ovarian Cancer Metastasis. Int J Mol Sci 23:N/A (2022). PubMed: 35055132