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AB244826

重组Anti-Anterior Gradient 2抗体[EPR20164-54] - BSA and Azide free (Capture)

Anti-Anterior Gradient 2 antibody [EPR20164-54] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal Anterior Gradient 2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

AG2, UNQ515/PRO1030, AGR2, Anterior gradient protein 2 homolog, AG-2, hAG-2, HPC8, Secreted cement gland protein XAG-2 homolog

1 Images
Sandwich ELISA - Anti-Anterior Gradient 2 antibody [EPR20164-54] - BSA and Azide free (Capture) (AB244826)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Anterior Gradient 2 antibody [EPR20164-54] - BSA and Azide free (Capture) (AB244826)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab240687).

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR20164-54

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Assay Kits

AB240687

Human AG-2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human AG-2 ELISA Kit (AB240687)

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  • 0
Sample Prep & Detection Kits

AB102893

PE/Cy5® Conjugation Kit - Lightning-Link®

Flow Cytometry - PE/Cy5® Conjugation Kit - Lightning-Link® (AB102893)

  • 0
  • 0
Sample Prep & Detection Kits

AB269893

Latex Conjugation Kit - 400nm Red

Functional Studies - Latex Conjugation Kit - 400nm Red (AB269893)

  • 0
  • 0
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Assay Kits

AB244185

Human AG-2 Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human AG-2 Antibody Pair - BSA and Azide free (AB244185)

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  • 0
Proteins & Peptides

AB64013

Recombinant Human Anterior Gradient 2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Anterior Gradient 2 protein (His tag N-Terminus) (AB64013)

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  • 0
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab244826 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human AG-2. The recommended pair for sandwich ELISA is:
Capture: ab244826, Human AG-2 Capture Antibody (unconjugated)
Detector: ab245099, Human AG-2 Detector Antibody (unconjugated)
The reference range value is 11.7 - 750 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Anterior Gradient 2 (AG2) also known as AGR2 is a protein with a molecular weight of approximately 20 kDa. This protein is a member of the protein disulfide isomerase family and has an important mechanical role in facilitating protein folding within the endoplasmic reticulum. The protein exists mainly in epithelial tissues and notably in the gastrointestinal tract with high expression also found in the pancreas and lungs. AG2 is characterized by its interaction with various client proteins assisting in their proper folding and stability.
Biological function summary

The anterior gradient protein influences cellular processes such as proliferation and differentiation often playing a role in cellular adhesion and migration. This protein does not work alone but instead can form complexes with other molecules enhancing its functional capabilities. Such interactions highlight its significance in maintaining normal cell physiology and response to external stimuli.

Pathways

Anterior gradient protein participates in key signaling pathways including the Wnt signaling pathway and the unfolded protein response (UPR). Through these pathways AG2 associates with proteins like β-catenin influencing gene transcription and cellular outcomes. In the UPR its role is important in managing cellular stress and maintaining homeostasis by regulating protein folding and quality control mechanisms.

Anterior gradient protein shows strong associations with cancer particularly in tumors such as breast and lung cancer where it is often overexpressed. Its involvement links closely with proteins like MUC1 which is found in tumors suggesting a role in promoting cancer progression and metastasis. Additionally AG2 can be related to inflammatory bowel diseases where its dysregulation may result in epithelial barrier dysfunction and contribute to the pathology.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion (PubMed : 23274113).
See full target information AGR2
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