AB250456

重组Anti-ANP32C/PP32R1抗体[EPR13354] - BSA and Azide free

Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ANP32C/PP32R1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

PP32R1, ANP32CP, Putative uncharacterized protein ANP32CP, Acidic leucine-rich nuclear phosphoprotein 32 family member C, Phosphoprotein 32-related protein 1, Tumorigenic protein pp32r1

4 Images

Flow Cytometry (Intracellular) - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (AB250456)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (AB250456)

This data was developed using ab181242, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed MCF-7 cells labeling ANP32C/PP32R1 with ab181242 at 1/20 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (AB250456)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (AB250456)

This data was developed using ab181242, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed PC-3 cells labeling ANP32C/PP32R1 with ab181242 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

Supplier Data

Immunoprecipitation - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (AB250456)

This data was developed using ab181242, the same antibody clone in a different buffer formulation.

Western blot analysis of ANP32C/PP32R1 in MCF-7 cell lysate immunoprecipitated with ab181242 at 1/50 dilution.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-ANP32C/PP32R1 antibody [EPR13354] (<a href='/products/primary-antibodies/anp32c-pp32r1-antibody-epr13354-ab181242'>ab181242</a>)

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

Supplier Data

Western blot - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (AB250456)

This data was developed using ab181242, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ANP32C/PP32R1 antibody [EPR13354] (<a href='/products/primary-antibodies/anp32c-pp32r1-antibody-epr13354-ab181242'>ab181242</a>) at 1/10000 dilution

Lane 1:

HUVEC cell lysate at 20 µg

Lane 2:

PC-3 cell lysate at 20 µg

Lane 3:

BxPC-3 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-ANP32C/PP32R1 antibody [EPR13354]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13354

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IP, Flow Cyt (Intra), WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab250456 is the carrier-free version of ab181242.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ANP32C also known as PP32R1 is a protein with a mass of approximately 30 kDa. It belongs to the acidic nuclear phosphoprotein 32 kDa family and plays mechanical roles in several cellular processes. ANP32C localizes mainly in the nucleus and shows expression in various tissues with notable presence in the brain and peripheral blood. Its functions involve interactions with other proteins to regulate cellular processes making it a significant target for research in cellular biology.

Biological function summary

ANP32C influences multiple cellular events including regulation of gene expression apoptosis and chromatin modification. It is part of several multiprotein complexes which contribute to its role in transcriptional regulation and cell cycle progression. The interaction of ANP32C with histone acetyltransferases plays a significant role in chromatin remodeling affecting gene expression patterns. This protein's actions are critical for maintaining normal cellular functions and responding to stress conditions.

Pathways

ANP32C engages in the apoptotic and inflammatory signaling pathways. It associates with proteins such as caspases and NF-kappa B influencing cell survival and immune responses. ANP32C's participation in the apoptotic pathway reflects its role in regulating programmed cell death a fundamental process for tissue homeostasis and development. Its interaction with inflammatory mediators highlights its contribution to immune responses and potential implications in inflammatory conditions.

ANP32C has connections to cancer and neurodegenerative diseases. Abnormal expression or mutation of ANP32C correlates with increased proliferation in some cancers emphasizing its role in tumorigenesis. Additionally the protein links with tau pathology implicating it in neurodegenerative disorders such as Alzheimer's disease. These associations suggest that ANP32C along with interacting proteins like tau may serve as potential therapeutic targets for disease intervention strategies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information ANP32CP

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao

View all publications

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Abcam Product Promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-ANP32C/PP32R1抗体[EPR13354] - BSA and Azide free