重组Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11抗体[EPR19442] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] - BSA and Azide free (AB251580)

This data was developed using ab223848, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Annexin A10 + Annexin A11 with ab223848 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and weak cytoplasmic staining in human bladder cancer (PMID : 24024557). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] - BSA and Azide free (AB251580)

This data was developed using ab223848, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Annexin A10 + Annexin A11 with ab223848 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and cytoplasmic staining in human stomach (PMID : 24024557; PMID : 21175800). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] - BSA and Azide free (AB251580)

This data was developed using ab223848, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling Annexin A10 + Annexin A11 with ab223848 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and weak cytoplasmic staining in human gastric cancer (PMID : 24024557; PMID : 21175800). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] - BSA and Azide free (AB251580)

This data was developed using ab223848, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Annexin A10 + Annexin A11 with ab223848 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and cytoplasmic staining in rat stomach. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• WB

Supplier Data

Western blot - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] - BSA and Azide free (AB251580)

This data was developed using ab223848, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-4, 6, and 7 : 3 minutes; Lane 5 : 30 seconds.

The molecular mass observed (35kDa) is consistent with what has been described in the literature (PMID : 21175800).

All lanes:

Western blot - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (<a href='/products/primary-antibodies/annexin-a10-anxa10-annexin-11-anxa11-antibody-epr19442-ab223848'>ab223848</a>) at 1/1000 dilution

Lane 1:

HT-1376 (human urinary bladder carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 3:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 5:

Human stomach lysate at 20 µg

Lane 6:

Human pancreas lysate at 20 µg

Lane 7:

BxPC-3 (human pancreas adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 5 - 7:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 37 kDa,54 kDa

Observed band size: 35 kDa,54 kDa

true

• WB

Supplier Data

Western blot - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] - BSA and Azide free (AB251580)

This data was developed using ab223848, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

This product cross-reacts with Annexin A11.

All lanes:

Western blot - Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (<a href='/products/primary-antibodies/annexin-a10-anxa10-annexin-11-anxa11-antibody-epr19442-ab223848'>ab223848</a>) at 1/10000 dilution

Lane 1:

His-tagged human Annexin A10 recombinant protein (aa134-324) at 0.01 µg

Lane 2:

His-tagged human Annexin A11 recombinant protein (aa1-505) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 37 kDa,54 kDa

Observed band size: 20 kDa,54 kDa

true

Exposure time: 30s