AB248814

重组Anti-ANKRD28抗体[EPR9211] - BSA and Azide free

Anti-ANKRD28 antibody [EPR9211] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ANKRD28 antibody. Carrier free. Suitable for WB and reacts with Human samples.

查看别名

KIAA0379, ANKRD28, Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A, PP6-ARS-A, Serine/threonine-protein phosphatase 6 regulatory subunit ARS-A, Ankyrin repeat domain-containing protein 28, Phosphatase interactor targeting protein hnRNP K, PITK

1 Images

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Western blot - Anti-ANKRD28 antibody [EPR9211] - BSA and Azide free (AB248814)

This data was developed using ab137091, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ANKRD28 antibody [EPR9211] - BSA and Azide free (ab248814) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Human fetal lung cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 113 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-ANKRD28 antibody [EPR9211]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR9211

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

ab248814 is the carrier-free version of ab137091.

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ANKRD28 also known as Ankyrin Repeat Domain 28 is a protein that contains several ankyrin repeat motifs which are known for mediating protein-protein interactions. It has a molecular mass of approximately 205 kDa. ANKRD28 is expressed in various tissues with higher expression observed in the brain and liver. The presence of ankyrin repeat motifs suggests that ANKRD28 plays a role in scaffolding or signaling facilitating the interaction between multiple proteins.

Biological function summary

ANKRD28 functions as a regulatory component that participates in cellular processes by forming complexes with other proteins. It is known to interact with protein phosphatase 6 (PP6) playing a role in signaling pathways and cellular regulation. Proper interaction within these complexes contributes to maintaining cellular homeostasis highlighting ANKRD28's importance in cellular signaling networks.

Pathways

ANKRD28 links to critical cellular mechanisms such as the NF-kB signaling pathway and stress response pathways. Within these pathways ANKRD28 modulates the function of downstream effector molecules including the phosphorylation status of IkB proteins which are essential for the regulation of NF-kB activity. ANKRD28 is related through these pathways to proteins like PP6 an important serine/threonine phosphatase involved in distributing cellular responses to various stimuli.

ANKRD28 has been associated with conditions like cancer and inflammatory diseases. In cancer altered expression or mutation of ANKRD28 can disrupt signaling pathways leading to uncontrolled cell growth and division. In inflammatory disorders dysregulation of NF-kB signaling potentially mediated by ANKRD28 contributes to excessive inflammatory responses. ANKRD28's interaction with proteins like I-kappaB kinase further connects it to disease progression affecting cellular processes that can lead to these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Putative regulatory subunit of protein phosphatase 6 (PP6) that may be involved in the recognition of phosphoprotein substrates. Involved in the PP6-mediated dephosphorylation of NFKBIE opposing its degradation in response to TNF-alpha. Selectively inhibits the phosphatase activity of PPP1C. Targets PPP1C to modulate HNRPK phosphorylation.

See full target information ANKRD28

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Abcam Product Promise

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详情请参阅我们的条款与条件。

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重组Anti-ANKRD28抗体[EPR9211] - BSA and Azide free