Anti-ANKRD1抗体(ab272894)
Key features and details
- Rabbit polyclonal to ANKRD1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ANKRD1抗体
参阅全部 ANKRD1 一抗 -
描述
兔多克隆抗体to ANKRD1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment within Human ANKRD1 (internal sequence). The exact sequence is proprietary.
Database link: Q15327 -
阳性对照
- WB: A431 and Jurkat whole cell lysate, mouse heart tissue lysate. IF: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272894于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. |
|
ICC/IF
1/100 - 1/1000. |
靶标
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功能
May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes. Induction seems to be correlated with apoptotic cell death in hepatoma cells. -
组织特异性
Mainly expressed in activated vascular endothelial cells. To a lower extent, also expressed in hepatoma cells. -
疾病相关
Defects in ANKRD1 may be a cause of total anomalous pulmonary venous return (TAPVR) [MIM:106700]. TAPVR is a rare congenital heart disease (CHD) in which the pulmonary veins fail to connect to the left atrium during cardiac development, draining instead into either the right atrium or one of its venous tributaries. This disease accounts for 1.5% of all CHDs and has a prevalence of approximately 1 out of 15'000 live births. -
序列相似性
Contains 5 ANK repeats. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 27063 Human
- Entrez Gene: 107765 Mouse
- Omim: 609599 Human
- SwissProt: Q15327 Human
- SwissProt: Q9CR42 Mouse
- Unigene: 448589 Human
- Unigene: 10279 Mouse
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别名
- ALRP antibody
- ANKR1_HUMAN antibody
- ANKRD 1 antibody
see all
图片
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Western blot - Anti-ANKRD1 antibody (ab272894)All lanes : Anti-ANKRD1 antibody (ab272894) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 30 µg per lane.10% SDS PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-ANKRD1 antibody (ab272894)A431 (human epidermoid carcinoma cell line) cells stained for ANKRD1 (Green) using ab2728/94 at 1/200 dilution in ICC/IF analysis. Counterstained with Hoechst 33342 (Blue).
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Western blot - Anti-ANKRD1 antibody (ab272894)Anti-ANKRD1 antibody (ab272894) at 1/1000 dilution + Mouse heart tissue lysate at 50 µg10% SDS PAGE
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab272894 被引用在 1 文献中.
- Qi L et al. Long Noncoding-RNA Component of Mitochondrial RNA Processing Endoribonuclease Promotes Carcinogenesis in Triple-Negative Breast Cancer Cells via the Competing Endogenous RNA Mechanism. J Breast Cancer 24:428-442 (2021). PubMed: 34652079