Anti-ANK-3抗体[N106/20] (ab134317)
Key features and details
- Mouse monoclonal [N106/20] to ANK-3
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-ANK-3抗体[N106/20]
参阅全部 ANK-3 一抗 -
描述
小鼠单克隆抗体[N106/20] to ANK-3 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Rat, Human -
免疫原
Fusion protein corresponding to Human ANK-3 (C terminal). Corresponding to 1000 C-terminal amino acids of human ANK-3, encompassing all of ANK-3 with the exception of Ankyrin repeats.
Database link: Q12955 -
阳性对照
- WB: Rat brain membrane lysate. ICC/IF: SK-N-BE cells.
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常规说明
The clone number has been updated from S106-20 to N106/20, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
N106/20 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134317于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 480 kDa.
1µg/ml of ab134317 is sufficient for detection of Ankyrin G in 20µg of rat brain membrane lysate, assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
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ICC/IF |
1/100.
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说明 |
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WB
1/1000. Predicted molecular weight: 480 kDa. 1µg/ml of ab134317 is sufficient for detection of Ankyrin G in 20µg of rat brain membrane lysate, assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
ICC/IF
1/100. |
靶标
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功能
In skeletal muscle, required for costamere localization of DMD and betaDAG1 (By similarity). Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. Regulates KCNA1 channel activity in function of dietary Mg(2+) levels, and thereby contributes to the regulation of renal Mg(2+) reabsorption (PubMed:23903368).
Isoform 5: May be part of a Golgi-specific membrane cytoskeleton in association with beta-spectrin. -
组织特异性
Expressed in brain, neurons, muscles and other tissues. -
疾病相关
Genetic variations in ANK3 may be associated with autism spectrum disorders susceptibility.
Mental retardation, autosomal recessive 37 -
序列相似性
Contains 23 ANK repeats.
Contains 1 death domain.
Contains 2 ZU5 domains. -
发展阶段
Up-regulated during muscle cell differentiation. -
结构域
The tandem configuration of the two ZU5 and the UPA domains forms a structural supramodule termed ZZU. ZU5-1 mediates interaction with beta-spectrin, and the ZU5-1/UPA interface is required for ankyrin's function other than binding to spectrin. -
细胞定位
Cytoplasm, cytoskeleton. Cell projection, axon. Cell membrane, sarcolemma. Cell junction, synapse, postsynaptic cell membrane. Lysosome. In skeletal muscle, localized at costameres and neuromuscular junctions. In macrophages, associated with lysosomes and Cytoplasm, cytoskeleton. Golgi apparatus. - Information by UniProt
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数据库链接
- Entrez Gene: 288 Human
- Entrez Gene: 361833 Rat
- Omim: 600465 Human
- SwissProt: Q12955 Human
- SwissProt: O70511 Rat
- Unigene: 499725 Human
- Unigene: 690023 Human
- Unigene: 731403 Human
see all -
别名
- ANK-3 antibody
- ANK3 antibody
- ANK3_HUMAN antibody
see all
图片
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4% formaldehyde-fixed SK-N-BE (human) cells labeling ANK-3 using ab134317 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/200 dilution incubated for 1 hour at rom temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).
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Western blot detection of ANK-3 in Rat brain membrane tissues using ab134317 at 1:1000 dilution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab134317 尚未被引用在任何文献中。